P2Y10/P2RY10 Overexpression Lysate (Native)

Images

 
Western Blot: P2Y10 Overexpression Lysate (Adult Normal) [NBL1-14035] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for P2Y10.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

P2Y10/P2RY10 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC220149 and based on accession number NM_198333. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens purinergic receptor P2Y, G-protein coupled, 10 (P2RY10), transcript variant 2, mRNA.
Gene
P2RY10

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
38.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for P2Y10/P2RY10

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for P2Y10/P2RY10 Overexpression Lysate (Native)

  • G-protein coupled purinergic receptor P2Y10
  • P2RY10
  • P2Y purinoceptor 10
  • P2Y10
  • P2Y10P2Y-like receptor
  • purinergic receptor P2Y, G-protein coupled, 10
  • putative P2Y purinoceptor 10

Background

The protein encoded by this gene belongs to the family of G-protein coupled receptors, that are preferentially activated by adenosine and uridine nucleotides. Two alternatively spliced transcript variants encoding the same protein isoform have been found for this gene. Transcript Variant: This variant (2) lacks 2 internal exons, as compared to variant 1. Both variants 1 and 2 encode the same isoform.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NLS4229
Species: Hu
Applications: ICC/IF, IHC-P
NLS418
Species: Hu
Applications: IHC-P
H00442206-R02
Species: Hu
H00441931-R02
Species: Hu
MAB194
Species: Hu, Mu
Applications: WB, Flow, CyTOF-ready, ICC
MAB8458
Species: Hu
Applications: Flow, CyTOF-ready, ICC
NBP1-02658
Species: Hu
Applications: ICC/IF (-), WB, IHC, IHC-P
NBP1-84903
Species: Hu
Applications: WB, IHC, IHC-P
NB110-39032
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-85740
Species: Hu, Po
Applications: WB, ICC/IF, IHC, IHC-P
AF-386-PB
Species: Hu
Applications: WB, Flow, CyTOF-ready, ICC
NBP1-90071
Species: Hu, Mu, Po, Eq
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-14197
Species: Hu
Applications: IHC, IHC-P
NBL1-14035
Species: Hu
Applications: WB

Publications for P2Y10/P2RY10 Lysate (NBL1-14035) (0)

There are no publications for P2Y10/P2RY10 Lysate (NBL1-14035).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for P2Y10/P2RY10 Lysate (NBL1-14035) (0)

There are no reviews for P2Y10/P2RY10 Lysate (NBL1-14035). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for P2Y10/P2RY10 Lysate (NBL1-14035) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional P2Y10/P2RY10 Products

Bioinformatics Tool for P2Y10/P2RY10 Lysate (NBL1-14035)

Discover related pathways, diseases and genes to P2Y10/P2RY10 Lysate (NBL1-14035). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for P2Y10/P2RY10 Lysate (NBL1-14035)

Discover more about diseases related to P2Y10/P2RY10 Lysate (NBL1-14035).

Research Areas for P2Y10/P2RY10 Lysate (NBL1-14035)

Find related products by research area.

Blogs on P2Y10/P2RY10

There are no specific blogs for P2Y10/P2RY10, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our P2Y10/P2RY10 Overexpression Lysate (Native) and receive a gift card or discount.

Bioinformatics

Gene Symbol P2RY10
Entrez