P2Y10/P2RY10 Antibody (639329) [Unconjugated]

HEK293 human embryonic kidney cell line transfected with P2Y10/P2RY10 and eGFP was stained with (A) Mouse Anti-Human P2Y10/P2RY10 Monoclonal Antibody (Catalog # MAB10158) or (B) Mouse IgG2B Isotype Control Antibody (Catalog # MAB0041) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

P2Y10/P2RY10 was detected in immersion fixed paraffin-embedded sections of human thymus using Mouse Anti-Human P2Y10/P2RY10 Monoclonal Antibody (Catalog # MAB10158) at 15 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

• Reactivity: Hu
• Applications: Flow, IHC, CyTOF-ready
• Clone: 639329
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

P2Y10/P2RY10 Antibody (639329) [Unconjugated] Summary

• Immunogen: NS0 mouse myeloma cell line transfected with human P2RY10
  Accession # O00398
• Specificity: Detects human P2RY10 in Flow Cytometry and Immunohistochemistry.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunohistochemistry 8-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for P2Y10/P2RY10 Antibody (639329) [Unconjugated]

• G-protein coupled purinergic receptor P2Y10
• P2RY10
• P2Y purinoceptor 10
• P2Y10
• P2Y10P2Y-like receptor
• purinergic receptor P2Y, G-protein coupled, 10
• putative P2Y purinoceptor 10

Background

P2Y receptors are a family of purinergic G protein-coupled receptors which belong to the class A family of GPCRs. They are membrane proteins, preferentially activated by nucleotides such as ADP, ATP, UTP, UDP and UDP-glucose. This P2Y receptor is thought to be a receptor for purines. While there is little known about the activity of this specific receptor, P2Y receptors mediate various biological functions such as vasodilation, blood clotting and immune responses.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for P2Y10/P2RY10 Antibody (MAB10158)(1)

Publication using MAB10158

• MS Sim, HJ Kim, SH Jo, C Kim, IY Chung Lysophosphatidylserine Induces MUC5AC Production via the Feedforward Regulation of the TACE-EGFR-ERK Pathway in Airway Epithelial Cells in a Receptor-Independent Manner International Journal of Molecular Sciences, 2022-03-31;23(7):. 2022-03-31 [PMID: 35409225] (Flow Cytometry, Human)

Bioinformatics

• Uniprot:
  • O00398()