P2Y1/P2RY1 Antibody

Product Discontinued

P2Y1/P2RY1 Antibody Summary

• Immunogen: Peptide with sequence C-KDLDNSPLRRK corresponding to internal region according to NP_002554.1.
• Epitope: KDLDNSPLRRK
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: P2RY1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:128000
• Application Notes: WB: Preliminary experiments gave bands at approx. 55 kDa band and 38 kDa band in lysates of HEK293 and A549 after 0.1 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 42.1 kDa band according to NP_002554.1. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the observed sizes).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Notes

Please note, this antibody is considered Innovators Grade. Innovators Grade antibodies are generally unvalidated and require additional characterization for most new species/applications. Novus has made these antibodies available through our Innovators Reward program. Complete an online review with image, detailing your positive or negative results. In return, you receive a discount voucher for 100% of the purchase price of the reviewed product. Please contact us at innovators@novusbio.com for more details.

Alternate Names for P2Y1/P2RY1 Antibody

• ATP receptor
• P2 purinoceptor subtype Y1
• P2RY1
• P2Y purinoceptor 1
• P2Y1
• P2Y1platelet ADP receptor
• purinergic receptor P2Y, G-protein coupled, 1
• Purinergic receptor

Background

The product of this gene belongs to the family of G-protein coupled receptors. This family has several receptor subtypes with different pharmacological selectivity, which overlaps in some cases, for various adenosine and uridine nucleotides. This receptor functions as a receptor for extracellular ATP and ADP. In platelets binding to ADP leads to mobilization of intracellular calcium ions via activation of phospholipase C, a change in platelet shape, and probably to platelet aggregation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for P2Y1/P2RY1 Antibody (NB100-79864)(1)

Publication using NB100-79864

• Arthur JF, Shen Y, Mu FT et al. Calmodulin interacts with the platelet ADP receptor P2Y1. Biochem J 2006-09-15 [PMID: 16848759]

Bioinformatics

• Gene Symbol: P2RY1
• Entrez:
  • 5028(Human)
  • 18441(Mouse)
  • 25265(Rat)
• Uniprot:
  • NP_002554.1(Human)