P2X6/P2RX6 Overexpression Lysate (Native)

Images

 
Western Blot: P2X6 Overexpression Lysate (Adult Normal) [NBL1-14032] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for P2X6.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

P2X6/P2RX6 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC206536 and based on accession number NM_005446. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens purinergic receptor P2X, ligand-gated ion channel, 6 (P2RX6), mRNA.
Gene
P2RX6

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
48.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for P2X6/P2RX6

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for P2X6/P2RX6 Overexpression Lysate (Native)

  • ATP receptor
  • MGC129625
  • P2RX6
  • P2RXL1
  • P2RXL1skeletal muscle-expressed P2X
  • P2X purinoceptor 6
  • P2X6
  • P2X6purinoceptor P2X6
  • P2XM
  • P2XMpurinoreceptor P2X6
  • purinergic receptor P2X, ligand-gated ion channel, 6
  • Purinergic receptor P2X-like 1
  • purinergic receptor P2X-like 1, orphan receptor
  • Purinergic receptor

Background

The protein encoded by this gene belongs to the family of P2X receptors, which are ATP-gated ion channels and mediate rapid and selective permeability to cations. This gene is predominantly expressed in skeletal muscle, and regulated by p53. The encoded protein is associated with VE-cadherin at the adherens junctions of human umbilical vein endothelial cells. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for P2X6/P2RX6 Lysate (NBL1-14032) (0)

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Bioinformatics

Gene Symbol P2RX6
Entrez