![Western Blot: OLLAS Epitope Tag Antibody (L2) [Biotin] [NBP1-06713B] - Oncohistone incorporation patterns induce changes in nuclear PRC2 distribution and sterility phenotypes. Western blot of H3K27me3 and H3.3/oncohistone levels in wild-type, mut, and H3-like mut worms. All versions of H3.3 are tagged with OLLAS Epitope Tag to distinguish them unambiguously form H3. H3 levels are shown as loading control. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-019-10404-9) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/OLLAS-Epitope-Tag-Antibody-L2-Biotin-Western-Blot-NBP1-06713B-img0002.jpg "Western Blot: OLLAS Epitope Tag Antibody (L2) [Biotin] [NBP1-06713B] - Oncohistone incorporation patterns induce changes in nuclear PRC2 distribution and sterility phenotypes. Western blot of H3K27me3 and H3.3/oncohistone levels in wild-type, mut, and H3-like mut worms. All versions of H3.3 are tagged with OLLAS Epitope Tag to distinguish them unambiguously form H3. H3 levels are shown as loading control. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-019-10404-9) licensed under a CC-BY license.")
| Reactivity | ETSpecies Glossary |
| Applications | WB, ICC/IF, IHC, IP, WB, ChIP, IHC |
| Clone | L2 |
| Clonality | Monoclonal |
| Host | Rat |
| Conjugate | Biotin |
| Immunogen | OLLAS (SGFANELGPRLMGK)-tagged extracellular domain of mouse Langerin |
| Isotype | IgG1 Kappa |
| Clonality | Monoclonal |
| Host | Rat |
| Purity | Protein G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | Optimal dilution of this antibody should be experimentally determined. |
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| Storage | Store at 4C. Do not freeze. |
| Buffer | PBS |
| Preservative | 0.05% Sodium Azide |
| Purity | Protein G purified |
| Publications using NBP1-06713B | Applications | Species |
|---|---|---|
| Wenda JM, Pros�e RF, Gabus C, Steiner FA. Mitotic chromosome condensation requires phosphorylation of the centromeric protein KNL-2 in C. elegans Journal of Cell Science 2021-12-01 [PMID: 34734636] (Chip Cytometry, Western Blot, Nematode - Caenorhabditis elegans) | Chip Cytometry, Western Blot | Nematode - Caenorhabditis elegans |
| Delaney K, Strobino M, Wenda JM et al H3.3K27M-induced chromatin changes drive ectopic replication through misregulation of the JNK pathway in C. elegans Nat Commun 2019-06-09 [PMID: 31175278] (Chip Cytometry, WB, C. elegans) | Chip Cytometry, WB | C. elegans |
| Images | Ratings | Applications | Species | Date | Details | ||||||||||
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Enlarge |
reviewed by:
Kamila Delaney |
ICC | C. elegans | 12/05/2017 |
Summary
Comments
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Secondary Antibodies |
Isotype Controls |
Research Areas for OLLAS Epitope Tag Antibody (NBP1-06713B)Find related products by research area.
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![Immunocytochemistry/Immunofluorescence: OLLAS Epitope Tag Antibody (L2) [Biotin] [NBP1-06713B] - Oncohistone incorporation patterns induce changes in nuclear PRC2 distribution and sterility phenotypes. Live cell imaging of GFP-tagged MES-2/EZH2 (the catalytic subunit of C. elegans PRC2), and immunofluorescence of H3K27me3 and H3.3/oncohistone (tagged with OLLAS Epitope Tag) in pachytene nuclei of H3-like K27M oncohistone (H3-like mut) worms. Scale bars represent 5 um. Chromosome X was identified by depletion of H3.3 and H3K4me3 staining shown in Supplementary Fig. 3, and is marked with an asterisk. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-019-10404-9) licensed under a CC-BY license](http://images.novusbio.com/fullsize/OLLAS-Epitope-Tag-Antibody-L2-Biotin-Immunocytochemistry-Immunofluorescence-NBP1-06713B-img0005.jpg "Immunocytochemistry/Immunofluorescence: OLLAS Epitope Tag Antibody (L2) [Biotin] [NBP1-06713B] - Oncohistone incorporation patterns induce changes in nuclear PRC2 distribution and sterility phenotypes. Live cell imaging of GFP-tagged MES-2/EZH2 (the catalytic subunit of C. elegans PRC2), and immunofluorescence of H3K27me3 and H3.3/oncohistone (tagged with OLLAS Epitope Tag) in pachytene nuclei of H3-like K27M oncohistone (H3-like mut) worms. Scale bars represent 5 um. Chromosome X was identified by depletion of H3.3 and H3K4me3 staining shown in Supplementary Fig. 3, and is marked with an asterisk. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-019-10404-9) licensed under a CC-BY license")
![Western Blot: OLLAS Epitope Tag Antibody (L2) [Biotin] [NBP1-06713B] - Oncohistone incorporation patterns induce changes in nuclear PRC2 distribution and sterility phenotypes. Western blot of H3K27me3 and H3.3/oncohistone levels in wild-type, mut, and H3-like mut worms. All versions of H3.3 are tagged with OLLAS Epitope Tag to distinguish them unambiguously form H3. H3 levels are shown as loading control. Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/s41467-019-10404-9) licensed under a CC-BY license.](https://images.novusbio.com/images/OLLAS-Epitope-Tag-Antibody-L2-Biotin-Western-Blot-NBP1-06713B-img0002.jpg "Western Blot: OLLAS Epitope Tag Antibody (L2) [Biotin] [NBP1-06713B] - Oncohistone incorporation patterns induce changes in nuclear PRC2 distribution and sterility phenotypes. Western blot of H3K27me3 and H3.3/oncohistone levels in wild-type, mut, and H3-like mut worms. All versions of H3.3 are tagged with OLLAS Epitope Tag to distinguish them unambiguously form H3. H3 levels are shown as loading control. Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/s41467-019-10404-9) licensed under a CC-BY license.")
