OLLAS Epitope Tag Antibody (L2)

Images

 
Western Blot: OLLAS Epitope Tag Antibody (L2) [NBP1-06713] - OLLAS tagged SFO-p41 protein.
Immunohistochemistry Whole-Mount: OLLAS Epitope Tag Antibody (L2) [NBP1-06713] - Drosophila adult midgut expressing an OLLAS-tagged protein stained with OLLAS Epitope Tag Antibody (magenta). Nuclei are shown in green. ...read more
Western Blot: OLLAS Epitope Tag Antibody (L2) [NBP1-06713] - Comparison of binding sensitivity of Novus Biologicals' monoclonal antibodies to OLLAS (NBP1-06713), DYKDDDDK (NBP1-06712) and the FLAG-M2 monoclonal antibody ...read more
OLLAS Epitope Tag Antibody (L2) [NBP1-06713] - Vector Map of pCMV-SD OLLAS

Product Details

Summary
Reactivity NASpecies Glossary
Applications WB, IB, ICC/IF, IHC, IHC-Fr, IP, IHC-WhMt
Clone
L2
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

OLLAS Epitope Tag Antibody (L2) Summary

Immunogen
OLLAS (SGFANELGPRLMGK)-tagged extracellular domain of mouse Langerin
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Rat
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:2000
  • Immunoblotting
  • Immunocytochemistry/Immunofluorescence 1:100
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Frozen 1:100
  • Immunoprecipitation 1:100-1:500
  • Immunohistochemistry Whole-Mount
Application Notes
This OLLAS Epitope Tag (L2) antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry on frozen sections, Immunoprecipitation and Western Blot. Use in immunoblotting reported in scientific literature (PMID: 27600537).
Reviewed Applications
Read 1 Review rated 4
using
NBP1-06713 in the following applications:

Publications
Read Publications using
NBP1-06713 in the following applications:

Reactivity Notes

Tag specific

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for OLLAS Epitope Tag Antibody (L2)

  • E.coli OmpF Linker and mouse Langerin fusion Sequence
  • OLLA-2

Background

OLLAS (Escherichia coli OmpF Linker and mouse Langerin fusion Sequence) epitope tag is a relatively newly discovered tag which is a 14 amino acid sequence residing in the junction between E. coli OmpF protein (OFL/OmpF linker) and mLangerin extracellular domain. OLLAS Epitope Tag antibody (L2), also called MAb L2 or OLLA-2, is a novel rat IgG monoclonal antibody raised against newly identified OLLAS epitope (SGFANELGPRLMGK), with advantages in immunodetection applications. Additionally, this antibody is a rat IgG and can be used for immunostaining of mouse and human samples without direct labelling of fluorochrome or enzyme. Because anti-tag antibodies are mostly generated from mouse and rabbit, the OLLAS Epitope Tag antibody (L2) from rat IgG provides an extra advantage in immunodetection.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for OLLAS Epitope Tag Antibody (NBP1-06713)(19)

We have publications tested in 8 confirmed species: Human, Mouse, Rat, C. elegans, C. elegens, Chinese Hamster, Drosophila, Parasite.

We have publications tested in 6 applications: ChIP-SEQ, IB, ICC/IF, IHC, IHC-Fr, WB.


Filter By Application
ChIP-SEQ
(1)
IB
(1)
ICC/IF
(4)
IHC
(2)
IHC-Fr
(1)
WB
(9)
All Applications
Filter By Species
Human
(3)
Mouse
(2)
Rat
(1)
C. elegans
(3)
C. elegens
(1)
Chinese Hamster
(1)
Drosophila
(2)
Parasite
(1)
All Species
Showing Publications 1 - 10 of 19. Show All 19 Publications.
Publications using NBP1-06713 Applications Species
Delaney K, Strobino M, Wenda JM et al H3.3K27M-induced chromatin changes drive ectopic replication through misregulation of the JNK pathway in C. elegans. Nat Commun. 2019 Jun 7 [PMID: 31175278] (WB, ChIP-SEQ, C. elegens)

Details:
Citation using the Biotin version of this antibody.
WB, ChIP-SEQ C. elegens
Chen YH, Narimatsu Y, Clausen TM et al. The GAGOme: a cell-based library of displayed glycosaminoglycans Nat. Methods Aug 13 2018 [PMID: 30104636] (ICC/IF) ICC/IF
Takemura M, Noborn F, Nilsson J et al. Chondroitin sulfate proteoglycan Windpipe modulates Hedgehog signaling in Drosophila. Int J Mol Sci. Oct 31 2018
Ouyang JPT, Folkmann A, Bernard L et al. P Granules Protect RNA Interference Genes from Silencing by piRNAs Dev. Cell Aug 6 2019 [PMID: 31402283] (C. elegans) C. elegans
Fujie T, Takenaka F, Yoshida E et al. A repetitive acidic region contributes to the extremely rapid degradation of the cell-context essential protein TRIM52 Sci Rep May 27 2019 [PMID: 31133683] (WB, Human) WB Human
Sales, EC;Heckman, EL;Warren, TL;Doe, CQ; Regulation of subcellular dendritic synapse specificity by axon guidance cues Elife Apr 23 2019 [PMID: 31012844] (IHC) IHC
Sen SQ, Chanchani S, Southall TD, Doe CQ. Neuroblast-specific open chromatin allows the temporal transcription factor, Hunchback, to bind neuroblast-specific loci Elife Jan 29 2019 [PMID: 30694180] (IHC) IHC
Haldeman JM, Conway AE, Arlotto ME et al. Creation of versatile cloning platforms for transgene expression and dCas9-based epigenome editing Nucleic Acids Res. Dec 27 2018 [PMID: 30590691] (WB, Rat) WB Rat
Huang TH, Niesman P, Arasu D et al. Tracing neuronal circuits in transgenic animals by transneuronal control of transcription (TRACT). Elife 2017 Dec 12 [PMID: 29231171] (Drosophila) Drosophila
Lee CS, Lu T, Seydoux G. Nanos promotes epigenetic reprograming of the germline by down-regulation of the THAP transcription factor LIN-15B. Elife 2017 Nov 07 [PMID: 29111977] (IHC-Fr, C. elegans) IHC-Fr C. elegans
Show All 19 Publications.

Review for OLLAS Epitope Tag Antibody (NBP1-06713) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Drosophila.

Reviews using NBP1-06713:
Filter by Applications
All Applications
Filter by Species
Drosophila
(1)
All Species
Images Ratings Applications Species Date Details
 OLLAS Epitope Tag NBP1-06713
Enlarge
reviewed by:
Masahiko Takemura
Drosophila 12/12/2016
View

Summary

Sample TestedDrosophila adult midgut
SpeciesDrosophila
LotF-1

Product General Protocols

View specific protocols for OLLAS Epitope Tag Antibody (NBP1-06713): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for OLLAS Epitope Tag Antibody (NBP1-06713). (Showing 1 - 2 of 2 FAQs).

  1. I am interested in tagging proteins from E. coli and am wondering if ompF cross-reactivity is a problem?
    • The OLLAS tag is not predicted to cross-react with any portion of ompF.
  2. Can you confirm if you measure the concentration of NBP1-06713 (anti-OLLAS epitope tag)?
    • Unfortunately, we do not measure the concentration of our OLLAS tag antibody, as the product is provided unpurified.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP1-06713AF350
Alexa Fluor 405 NBP1-06713AF405
Alexa Fluor 488 NBP1-06713AF488
Alexa Fluor 532 NBP1-06713AF532
Alexa Fluor 594 NBP1-06713AF594
Alexa Fluor 647 NBP1-06713AF647
Alexa Fluor 700 NBP1-06713AF700
Alexa Fluor 750 NBP1-06713AF750
Biotin NBP1-06713B
DyLight 350 NBP1-06713UV
DyLight 405 NBP1-06713V
DyLight 488 NBP1-06713G
DyLight 550 NBP1-06713R
DyLight 594 NBP1-06713DL594
DyLight 650 NBP1-06713C
DyLight 680 NBP1-06713FR
DyLight 755 NBP1-06713IR
FITC NBP1-06713F
HRP NBP1-06713H
Janelia Fluor 549 NBP1-06713JF549
Janelia Fluor 646 NBP1-06713JF646

Additional OLLAS Epitope Tag Products

Array NBP1-06713

Research Areas for OLLAS Epitope Tag Antibody (NBP1-06713)

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Blogs on OLLAS Epitope Tag

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Recent Reviews

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Masahiko Takemura
12/12/2016
Application:
Species: Drosophila