OLLAS Epitope Tag Antibody (L2) [mFluor Violet 450 SE] Summary
| Immunogen |
OLLAS (SGFANELGPRLMGK)-tagged extracellular domain of mouse Langerin |
| Isotype |
IgG1 Kappa |
| Clonality |
Monoclonal |
| Host |
Rat |
| Purity |
Protein G purified |
| Innovator's Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase. |
Applications/Dilutions
| Dilutions |
- Chromatin Immunoprecipitation Sequencing
- Immunoblotting
- Immunocytochemistry/ Immunofluorescence
- Immunohistochemistry Whole-Mount
- Immunohistochemistry
- Immunohistochemistry-Frozen
- Immunohistochemistry-Paraffin
- Immunoprecipitation
- Western Blot
|
| Application Notes |
Optimal dilution of this antibody should be experimentally determined. |
Packaging, Storage & Formulations
| Storage |
Store at 4C in the dark. |
| Buffer |
50mM Sodium Borate |
| Preservative |
0.05% Sodium Azide |
| Purity |
Protein G purified |
Notes
mFluor(TM) is a trademark of AAT Bioquest, Inc. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.
Alternate Names for OLLAS Epitope Tag Antibody (L2) [mFluor Violet 450 SE]
Background
The OLLAS (E.coli OmpF Linker and mouse Langerin fusion Sequence) epitope tag is a newly defined tag that is 100-fold more sensitive to other epitope tags tested, including Sigma's anti-FLAG(R) epitope tag antibody. OLLAS is a far superior tag for immunoprecipitation, fluorescent immunohistochemistry and flow cytometry detection.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are
guaranteed for 1 year from date of receipt.
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Product General Protocols
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Video Protocols
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Secondary Antibodies
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Isotype Controls
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Additional OLLAS Epitope Tag Products
Blogs on OLLAS Epitope Tag
![Western Blot: OLLAS Epitope Tag Antibody (L2) [Biotin] [NBP1-06713B] - Oncohistone incorporation patterns induce changes in nuclear PRC2 distribution and sterility phenotypes. Western blot of H3K27me3 and H3.3/oncohistone levels in wild-type, mut, and H3-like mut worms. All versions of H3.3 are tagged with OLLAS Epitope Tag to distinguish them unambiguously form H3. H3 levels are shown as loading control. Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/s41467-019-10404-9) licensed under a CC-BY license.](https://images.novusbio.com/images/OLLAS-Epitope-Tag-Antibody-L2-Biotin-Western-Blot-NBP1-06713B-img0002.jpg "Western Blot: OLLAS Epitope Tag Antibody (L2) [Biotin] [NBP1-06713B] - Oncohistone incorporation patterns induce changes in nuclear PRC2 distribution and sterility phenotypes. Western blot of H3K27me3 and H3.3/oncohistone levels in wild-type, mut, and H3-like mut worms. All versions of H3.3 are tagged with OLLAS Epitope Tag to distinguish them unambiguously form H3. H3 levels are shown as loading control. Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/s41467-019-10404-9) licensed under a CC-BY license.")