OLLAS Epitope Tag Antibody (L2) - Azide and BSA Free

Western Blot: OLLAS Epitope Tag Antibody (L2) - Azide and BSA Free [NBP2-80893] - OLLAS tagged SFO-p41 protein. Image from the standard format of this antibody.

Immunocytochemistry/Immunofluorescence: OLLAS Epitope Tag Antibody (L2) - Azide and BSA Free [NBP2-80893] - Oncohistone incorporation patterns induce changes in nuclear PRC2 distribution and sterility phenotypes. Live cell imaging of GFP-tagged MES-2/EZH2 (the catalytic subunit of C. elegans PRC2), and immunofluorescence of H3K27me3 and H3.3/oncohistone (tagged with OLLAS Epitope Tag) in pachytene nuclei of H3-like K27M oncohistone (H3-like mut) worms. Scale bars represent 5 um. Chromosome X was identified by depletion of H3.3 and H3K4me3 staining shown in Supplementary Fig. 3, and is marked with an asterisk. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-019-10404-9) licensed under a CC-BY license Image using the Biotin format of this antibody. Image from the standard format of this antibody.

Immunohistochemistry: OLLAS Epitope Tag Antibody (L2) - Azide and BSA Free [NBP2-80893] - Different intracellular and transmembrane domains (from Nrx, nSyb, and sdc) were fused to CD19 and expressed into ORNs targeting the DA1 glomerulus using the CD19::Nrx (top), nSyb::CD19 (middle) and CD19::Sdc (bottom), with the R17H02 driver (in green). T

Western Blot: OLLAS Epitope Tag Antibody (L2) - Azide and BSA Free [NBP2-80893] - C. elegans whole nematode. OLLAS::PRMT-1 Western Blot using NBP1-06713SS (1:1000). Western blot image submitted by a verified customer review. Image from the standard format of this antibody.

Western Blot: OLLAS Epitope Tag Antibody (L2) - Azide and BSA Free [NBP2-80893] - Comparison of binding sensitivity of Novus Biologicals' monoclonal antibodies to OLLAS (NBP1-06713), DYKDDDDK (NBP1-06712) and the FLAG-M2 monoclonal antibody from Sigma-Aldrich. FLAG (TM) and ANTI-FLAG (TM) are registered trademarks of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co. Image from the standard format of this antibody.

Immunohistochemistry: OLLAS Epitope Tag Antibody (L2) - Azide and BSA Free [NBP2-80893] - Drosophila adult midgut expressing an OLLAS-tagged protein stained with OLLAS Epitope Tag Antibody (magenta). Nuclei are shown in green. Image from verified customer review. Image from the standard format of this antibody.

Immunohistochemistry: OLLAS Epitope Tag Antibody (L2) - Azide and BSA Free [NBP2-80893] - MES proteins are expressed in nos-1nos-2 embryonic PGCs.Transcriptome comparison between PGCs isolated from wild-type and mes-2(RNAi) L1 larvae. Top: Photomicrograph of live embryo expressing GFP tagged MES-2 in wild- type and nos-1(gv5)nos-2(ax3103) embr

• Reactivity: ET
• Applications: WB, ICC/IF, IHC, IP, WB, IHC
• Clone: L2
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

OLLAS Epitope Tag Antibody (L2) - Azide and BSA Free Summary

• Description: Novus Biologicals Rat OLLAS Epitope Tag Antibody (L2) - Azide and BSA Free (NBP1-06713) is a monoclonal antibody validated for use in IHC, WB, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: OLLAS (SGFANELGPRLMGK)-tagged extracellular domain of mouse Langerin
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Rat
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunoblotting reported in scientific literature (PMID 27600537)
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry Whole-Mount
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Frozen 1:100
  • Immunoprecipitation 1:100-1:500
  • Western Blot 1:1000-1:2000

Reactivity Notes

Tag specific

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: Protein G purified

Alternate Names for OLLAS Epitope Tag Antibody (L2) - Azide and BSA Free

• E.coli OmpF Linker and mouse Langerin fusion Sequence
• OLLA-2

Background

OLLAS (Escherichia coli OmpF Linker and mouse Langerin fusion Sequence) epitope tag is a relatively newly discovered tag which is a 14 amino acid sequence residing in the junction between E. coli OmpF protein (OFL/OmpF linker) and mLangerin extracellular domain. OLLAS Epitope Tag antibody (L2), also called MAb L2 or OLLA-2, is a novel rat IgG monoclonal antibody raised against newly identified OLLAS epitope (SGFANELGPRLMGK), with advantages in immunodetection applications. Additionally, this antibody is a rat IgG and can be used for immunostaining of mouse and human samples without direct labelling of fluorochrome or enzyme. Because anti-tag antibodies are mostly generated from mouse and rabbit, the OLLAS Epitope Tag antibody (L2) from rat IgG provides an extra advantage in immunodetection.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.