Recombinant Human OAS1 His Protein

SDS-Page: Recombinant Human OAS1 Protein [NBP1-30214] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

• Reactivity: Hu
• Applications: PAGE
• Concentration: 1 mg/ml

Recombinant Human OAS1 His Protein Summary

• Description: A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-364 of Human OAS1
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MMDLRNTPAK SLDKFIEDYL LPDTCFRMQI NHAIDIICGF LKERCFRGSS YPVCVSKVVK GGSSGKGTTL RGRSDADLVV FLSPLTTFQD QLNRRGEFIQ EIRRQLEACQ RERAFSVKFE VQAPRWGNPR ALSFVLSSLQ LGEGVEFDVL PAFDALGQLT GSYKPNPQIY VKLIEECTDL QKEGEFSTCF TELQRDFLKQ RPTKLKSLIR LVKHWYQNCK KKLGKLPPQY ALELLTVYAW ERGSMKTHFN TAQGFRTVLE LVINYQQLCI YWTKYYDFKN PIIEKYLRRQ LTKPRPVILD PADPTGNLGG GDPKGWRQLA QEAEAWLNYP CFKNWDGSPV SSWILLVRPP ASSLPFIPAP LHEA

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: OAS1
• Purity: >95%, by SDS-PAGE
• Endotoxin Note: < 1.0 EU per 1 microgram of protein (determined by LAL method)

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 43.9 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH 8.0), 10% glycerol, 1 mM DTT
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: >95%, by SDS-PAGE

Alternate Names for Recombinant Human OAS1 His Protein

• (2-5')oligo(A) synthase 1,2'-5'-oligoadenylate synthetase 1,2-5A synthase 1,2'-5'-oligoisoadenylate synthetase 1
• (2-5')oligo(A) synthetase 1
• 2'-5'-oligo A synthetase 1
• 2'-5'-oligoadenylate synthase 1
• 2'-5'-oligoadenylate synthetase 1 (40-46 kD)
• 2'-5'-oligoadenylate synthetase 1, 40/46kDa
• E18/E16,2-5A synthetase 1
• EC 2.7.7
• EC 2.7.7.-
• IFI-4,2'-5' oligoadenylate synthetase 1 p52 isoform
• OIAS2'-5' oligoadenylate synthetase 1 p48 isoform
• OIASI
• p46/p42 OAS

Background

OAS1 is an enzyme included in the 2',5'-oligoadenylate synthase family. This enzyme is induced by interferons and uses adenosine triphosphate in 2'-specific nucleotidyl transfer reactions to synthesize 2',5'-oligoadenylates(2-5As). These molecules activate latent RNase L, which results in viral RNA degradation and the inhibition of viral replication. OAS1 may play a role in mediating resistance to virus infection, control of cell growth, differentiation, and apoptosis. Recombinant OAS1 protein was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: OAS1