OAS1 Overexpression Lysate (Native) Summary
The lysate was created in HEK293T cells, using plasmid ID RC200696 and based on accession number NM_002534. The protein contains a C-terminal DDK tag.
Homo sapiens 2',5'-oligoadenylate synthetase 1, 40/46kDa (OAS1), transcript variant 2, mRNA.
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Packaging, Storage & Formulations
Store at -80C. Avoid freeze-thaw cycles.
Lysate Details for OAS1
HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.
Alternate Names for OAS1 Overexpression Lysate (Native)
- (2-5')oligo(A) synthase 1,2'-5'-oligoadenylate synthetase 1,2-5A synthase 1,2'-5'-oligoisoadenylate synthetase 1
- (2-5')oligo(A) synthetase 1
- 2'-5'-oligo A synthetase 1
- 2'-5'-oligoadenylate synthase 1
- 2'-5'-oligoadenylate synthetase 1 (40-46 kD)
- 2'-5'-oligoadenylate synthetase 1, 40/46kDa
- E18/E16,2-5A synthetase 1
- EC 2.7.7
- EC 2.7.7.-
- IFI-4,2'-5' oligoadenylate synthetase 1 p52 isoform
- OIAS2'-5' oligoadenylate synthetase 1 p48 isoform
- p46/p42 OAS
This gene encodes a member of the 2-5A synthetase family, essential proteins involved in the innate immune response to viral infection. The encoded protein is induced by interferons and uses adenosine triphosphate in 2'-specific nucleotidyl transfer reactions to synthesize 2',5'-oligoadenylates (2-5As). These molecules activate latent RNase L, which results in viral RNA degradation and the inhibition of viral replication. The three known members of this gene family are located in a cluster on chromosome 12. Mutations in this gene have been associated with host susceptibility to viral infection. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq]. Transcript Variant: This variant (2) contains an additional segment in the 3' coding region, compared to variant 1, which leads to a premature stop codon. It encodes isoform 2, also known as E16 and p40 and p42, which has a shorter, distinct C-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed
for 6 months from date of receipt.
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