OAS1 Overexpression Lysate (Native) (NBL1-13896)

Images

 
Western Blot: OAS1 Overexpression Lysate (Adult Normal) [NBL1-13896] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for OAS1.

Product Details

Summary
Applications WB

Order Details

OAS1 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC200696 and based on accession number NM_002534. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens 2',5'-oligoadenylate synthetase 1, 40/46kDa (OAS1), transcript variant 2, mRNA.
Gene
OAS1

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
41.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for OAS1

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for OAS1 Overexpression Lysate (Native)

  • (2-5')oligo(A) synthase 1,2'-5'-oligoadenylate synthetase 1,2-5A synthase 1,2'-5'-oligoisoadenylate synthetase 1
  • (2-5')oligo(A) synthetase 1
  • 2'-5'-oligo A synthetase 1
  • 2'-5'-oligoadenylate synthase 1
  • 2'-5'-oligoadenylate synthetase 1 (40-46 kD)
  • 2'-5'-oligoadenylate synthetase 1, 40/46kDa
  • E18/E16,2-5A synthetase 1
  • EC 2.7.7
  • EC 2.7.7.-
  • IFI-4,2'-5' oligoadenylate synthetase 1 p52 isoform
  • OIAS2'-5' oligoadenylate synthetase 1 p48 isoform
  • OIASI
  • p46/p42 OAS

Background

This gene encodes a member of the 2-5A synthetase family, essential proteins involved in the innate immune response to viral infection. The encoded protein is induced by interferons and uses adenosine triphosphate in 2'-specific nucleotidyl transfer reactions to synthesize 2',5'-oligoadenylates (2-5As). These molecules activate latent RNase L, which results in viral RNA degradation and the inhibition of viral replication. The three known members of this gene family are located in a cluster on chromosome 12. Mutations in this gene have been associated with host susceptibility to viral infection. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq]. Transcript Variant: This variant (2) contains an additional segment in the 3' coding region, compared to variant 1, which leads to a premature stop codon. It encodes isoform 2, also known as E16 and p40 and p42, which has a shorter, distinct C-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-47859
Species: Hu, Mu, Mk
Applications: WB, ICC/IF
AF5550
Species: Mu, Rt
Applications: WB
MAB1925
Species: Hu
Applications: IP, ICC
NB100-351
Species: Hu, Ha, Pm, Mu(-), Rt(-)
Applications: WB, ELISA, IHC, IHC-P
NB100-64387
Species: Mu
Applications: B/N, ELISA, Func, IHC, IHC-Fr
NBP1-48011
Species: Hu, Ca, Pm
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-85841
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-87363
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-77266
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF, IHC, IHC-P, IF
AF8519
Species: Hu, Mu, Rt
Applications: WB
NBP2-02340
Species: Hu, Mu, Rt, Mk
Applications: WB, Flow, ICC/IF, IHC, IHC-P
AF5629
Species: Hu, Mu
Applications: WB
NBP2-24875
Species: Hu, Mu, Ca
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP, Flow-IC
NBP2-43581
Species: Hu
Applications: WB, ICC/IF, IP
NBP2-27109
Species: Hu, Mu, Po, Pm
Applications: WB, Simple Western, IHC, IHC-P
NBP1-77263
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF, IHC, IHC-P

Publications for OAS1 Lysate (NBL1-13896) (0)

There are no publications for OAS1 Lysate (NBL1-13896).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for OAS1 Lysate (NBL1-13896) (0)

There are no reviews for OAS1 Lysate (NBL1-13896). By submitting a review earn points towards our Rewards Program.
  • 250 points for product review
  • 500 additional points for an image with your product review
  • Double points (500) if you are the first to review this product
  • Double points (1000) if you are the first to review this product with an image

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for OAS1 Lysate (NBL1-13896) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional OAS1 Products

Related Products by Gene

Bioinformatics Tool for OAS1 Lysate (NBL1-13896)

Discover related pathways, diseases and genes to OAS1 Lysate (NBL1-13896). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for OAS1 Lysate (NBL1-13896)

Discover more about diseases related to OAS1 Lysate (NBL1-13896).
 

Pathways for OAS1 Lysate (NBL1-13896)

View related products by pathway.

PTMs for OAS1 Lysate (NBL1-13896)

Learn more about PTMs related to OAS1 Lysate (NBL1-13896).
 

Research Areas for OAS1 Lysate (NBL1-13896)

Find related products by research area.

Blogs on OAS1

There are no specific blogs for OAS1, but you can read our latest blog posts.

Customers Who Bought This Also Bought

OAS1 Antibody
NBP1-83122
OAS1 Antibody
NBP1-83122
OAS1 Antibody
NBP1-83122

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our OAS1 Overexpression Lysate (Native) and receive a gift card or discount.

Bioinformatics

Gene Symbol OAS1