NDUFS8 Antibody

Western Blot: NDUFS8 Antibody [NBP1-49847] - Western blot analysis of NDUFS8 in Mouse Heart lysate (35ug protein in RIPA buffer) using NBP1-49847 at 0.1ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Mu, Rt, Hu, Ca
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

NDUFS8 Antibody Summary

• Description: Novus Biologicals Goat NDUFS8 Antibody (NBP1-49847) is a polyclonal antibody validated for use in WB and ELISA. Anti-NDUFS8 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-EAEIAANIQAD corresponding to internal region according to NP_002487.1.
• Epitope: EAEIAANIQAD - internal region
• Predicted Species: Human (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: NDUFS8
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:4000
  • Western Blot 0.1 - 0.3 ug/ml
• Application Notes: WB: Approx. 25 kDa band observed in mouse and rat heart lysates (calculated MW of 24.0 kDa band according to mouse NP_659119.2 and rat NP_001099792.1).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for NDUFS8 Antibody

• CI-23k
• CI23KD
• CI-23kD
• complex I 23kDa subunit
• Complex I-23kD
• EC 1.6.5.3
• EC 1.6.99.3
• EC 1.6.99.5
• NADH dehydrogenase (ubiquinone) Fe-S protein 8 (23kD) (NADH-coenzyme Qreductase)
• NADH dehydrogenase (ubiquinone) Fe-S protein 8, 23kDa (NADH-coenzyme Qreductase)
• NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial
• NADH-ubiquinone oxidoreductase 23 kDa subunit
• TYKY subunit
• TYKY

Background

NDUFS8 (NADH dehydrogenase (ubiquinone) Fe-S protein 8, 23kDa (NADH-coenzyme Q reductase)) is a subunit of NADH. Mutations in NDUFS8 have been linked to Leigh syndrome.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NDUFS8 Antibody (NBP1-49847)(1)

Publication using NBP1-49847

• Saito A, Kawamoto M, Kamatani N. Association study between single-nucleotide polymorphisms in 199 drug-related genes and commonly measured quantitative traits of 752 healthy Japanese subjects. J Hum Genet 2009-06-01 [PMID: 19343046]

Bioinformatics

• Gene Symbol: NDUFS8
• Entrez:
  • 4728(Human)
  • 225887(Mouse)
  • 293652(Rat)
• Uniprot:
  • NP_002487.1(Human)