Napsin A Antibody (KCG1.1)

Immunohistochemistry-Paraffin: Napsin A Antibody (KCG1.1) [NB110-90605] - Formalin fixed paraffin embedded human Lung adenocarcinoma stained with Napsin A antibody (NB110-90605).

Immunohistochemistry-Paraffin: Napsin A Antibody (KCG1.1) [NB110-90605] - Formalin fixed paraffin embedded human lung adenocarcinoma stained with Napsin A monoclonal antibody.

Product Discontinued

Napsin A Antibody (KCG1.1) Summary

• Immunogen: BALB/C mice were injected with synthetic peptide from N terminus of human Napsin A.
• Localization: Cytoplasmic
• Specificity: Napsin is found in two isoforms, A and B, with highly homologous gene sequences. Napsin A has a molecular weight of 35.0 kDa and is also known as TAO2. Napsin A is an aspartic proteinase which is expressed in the lung and involved in processing surfactant protein B (SP-B). It is also expressed in the kidney. This antibody may be a useful tool as a tumor marker for primary lung adenocarcinoma. Napsin expression correlates with the differentiation grade of lung adenocarcinoma.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: NAPSA
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:50-1:100
  • Immunohistochemistry-Paraffin
• Application Notes: IHC-P: recommended pretreatment of citrate buffer, pH 6.0. Recommended incubation time of 30 min at RT.
• Control:

  Napsin A Overexpression Lysate

Reactivity Notes

Human.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.4), 0.2% BSA, Tween-20
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified

Alternate Names for Napsin A Antibody (KCG1.1)

• Asp 4
• ASP4
• Aspartyl protease 4
• EC 3.4.23
• EC 3.4.23.-
• EC 3.4.23.15
• EC 3.4.23.3
• EC 3.4.23.5
• KAP
• Kdap
• NAP1napsin-A
• NAPA
• NAPApronapsin A
• NAPSA
• napsin A aspartic peptidase
• Napsin A
• Napsin-1
• SNAPA
• TA01/TA02

Background

Napsin is a pepsin-like aspartic proteinase, in the A1 clan of the AA clade of proteinases. Other AA family proteinases include Pepsin, cathepsin-D, cathepsin-E and rennin. Of these proteinases, napsin is most closely related to cathepsin-D, sharing 46% identity at the amino acid level. There are two closely related Napsins, napsin A and napsin B, with 85% overall identity at the amino acid level. There are three isoforms. Napsin-A is also termed napsin-1, or TA02, a Tumor Adenocarcinoma marker. Napsin A may be involved in processing of pneumocyte surfactant precursors.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NAPSA
• Entrez:
  • 9476(Human)
• Uniprot:
  • O96009()