Recombinant Bacteria NADK His Protein

• Reactivity: Ba
• Applications: Enzyme Activity

Recombinant Bacteria NADK His Protein Summary

• Description: NAD kinase (Mycobacterium tuberculosis) fused at the N-terminus to a His-tag.
  
  Source: E. coli

  Uniprot ID: P0A5S6

• Details of Functionality: ~1.2 U/mg protein (Substrate: NAD (+ ATP))
  ~3.8 U/mg protein (Substrate: NAD (+ poly(P))
  One unit is defined as the amount of enzyme that synthesizes 1umol nicotinamide adenine dinucleotide phosphate (NADP) per min
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: NADK
• Purity: >98%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • Enzyme Activity
• Theoretical MW: 33 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

M. tuberculosis

Packaging, Storage & Formulations

• Storage: Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
• Buffer: 10mM TRIS at pH 8.0, 1mM MgCl2, 0.5mM EDTA, 1mM DTT and 1mM PMSF
• Preservative: No Preservative
• Purity: >98%, by SDS-PAGE

Alternate Names for Recombinant Bacteria NADK His Protein

• dJ283E3.1
• DKFZp686L22239
• EC 2.7.1
• EC 2.7.1.23
• FLJ13052
• FLJ37724
• FLJ54695
• FLJ77769
• FLJ78247
• FLJ78307
• MGC1900
• NAD kinase
• NADK
• Poly(P)/ATP NAD kinase

Background

NADK catalyzes the transfer of a phosphate group from ATP to NAD to generate NADP, which in its reduced form acts as an electron donor for biosynthetic reactions (Lerner et al., 2001 [PubMed 11594753]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: NADK
• Uniprot:
  • P0A5S6(Bacteria)