Recombinant B. subtilis NADK Protein

• Reactivity: Ba
• Applications: Enzyme Activity

Recombinant B. subtilis NADK Protein Summary

• Description: Full-length NAD kinase (Bacillus subtilis).
  
  Source: E. coli

  Uniprot ID: O31612

• Details of Functionality: ~2 6 U/mg protein One unit is defined as the amount of enzyme that synthesizes 1umol nicotinamide adenine dinucleotide phosphate (NADP) per min
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: NADK
• Purity: >98%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • Enzyme Activity
• Theoretical MW: 30 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
• Buffer: 50mM TRIS-HCl, pH 7.0, 150mM NaCl, 1mM EDTA and 1mM dithiothreitol
• Preservative: No Preservative
• Purity: >98%, by SDS-PAGE

Alternate Names for Recombinant B. subtilis NADK Protein

• dJ283E3.1
• DKFZp686L22239
• EC 2.7.1
• EC 2.7.1.23
• FLJ13052
• FLJ37724
• FLJ54695
• FLJ77769
• FLJ78247
• FLJ78307
• MGC1900
• NAD kinase
• NADK
• Poly(P)/ATP NAD kinase

Background

NADK catalyzes the transfer of a phosphate group from ATP to NAD to generate NADP, which in its reduced form acts as an electron donor for biosynthetic reactions (Lerner et al., 2001 [PubMed 11594753]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: NADK
• Uniprot:
  • O31612(B. subtilis)