MYST1 Antibody (JG36-05)

Immunocytochemistry/Immunofluorescence: MYST1 Antibody (JG36-05) [NBP2-76844] - ICC staining KAT8 in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry: MYST1 Antibody (JG36-05) [NBP2-76844] - Immunohistochemical analysis of paraffin-embedded rat cervix tissue using anti-KAT8 antibody. Counter stained with hematoxylin.

Flow Cytometry: MYST1 Antibody (JG36-05) [NBP2-76844] - Flow cytometric analysis of K562 cells with KAT8 antibody at 1/100 dilution (yellow) compared with an unlabelled control (cells without incubation with primary antibody; purple).Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

Immunocytochemistry/Immunofluorescence: MYST1 Antibody (JG36-05) [NBP2-76844] - ICC staining KAT8 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: MYST1 Antibody (JG36-05) [NBP2-76844] - ICC staining KAT8 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry: MYST1 Antibody (JG36-05) [NBP2-76844] - Immunohistochemical analysis of paraffin-embedded human cervix tissue using anti-KAT8 antibody. Counter stained with hematoxylin.

Immunohistochemistry: MYST1 Antibody (JG36-05) [NBP2-76844] - Immunohistochemical analysis of paraffin-embedded human cervix cancer tissue using anti-KAT8 antibody. Counter stained with hematoxylin.

Product Discontinued

MYST1 Antibody (JG36-05) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Recombinant protein within Human MYST1 aa 310-458 / 458. (SwissProt: Q9H7Z6 Human; SwissProt: Q9D1P2 Mouse; SwissProt: Q5XI06 Rat)
• Localization: Chromosome. Nucleus.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: KAT8
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500
• Theoretical MW: 53 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for MYST1 Antibody (JG36-05)

• FLJ14040
• hMOFhistone acetyltransferase MYST1
• KAT8
• MOFEC 2.3.1.48
• MOZ, YBF2/SAS3, SAS2 and TIP60 protein 1
• MYST histone acetyltransferase 1
• MYST-1
• ortholog of Drosophila males absent on the first (MOF)
• probable histone acetyltransferase MYST1

Background

The MYST family of histone acetyltransferases, which includes MYST1, is named for the founding members MOZ (MYST3; MIM 601408), yeast YBF2 and SAS2, and TIP60 (HTATIP; MIM 601409). All members of this family contain a MYST region of about 240 amino acids with a canonical acetyl-CoA-binding site and a C2HC-type zinc finger motif. Most MYST proteins also have a chromodomain involved in protein-protein interactions and targeting transcriptional regulators to chromatin (Neal et al., 2000).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: KAT8