MYST1 Antibody (8C4C4)

Western Blot: MYST1 Antibody (8C4C4) [NBP1-28879] - Analysis using MYST1 mouse mAb against Hela (1), HepG2 (2) and SMMC-7721 (3) cell lysate.

Immunocytochemistry/Immunofluorescence: MYST1 Antibody (8C4C4) [NBP1-28879] - Analysis of Eca 109 cells using MOF/MYST1 mouse mAb (green), showing nuclear localization.

Immunohistochemistry-Paraffin: MYST1 Antibody (8C4C4) [NBP1-28879] - Analysis of human esophageal squamous cell carcinoma (A), normal esophagus epithelium (B), rectum adenocarcinoma (C), lung squamous cell carcinoma (D), breast infiltrating carcinoma (E), and breast infiltrating carcinoma (F) tissues, showing nuclear local

Product Discontinued

MYST1 Antibody (8C4C4) Summary

• Immunogen: Purified recombinant fragment of human MYST1 expressed in E. Coli.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: KAT8
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Immunocytochemistry/ Immunofluorescence 1:200 - 1:1000
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:1000
  • Western Blot 1:500 - 1:2000
• Theoretical MW: 53 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: 0.03% Sodium Azide
• Purity: Unpurified

Alternate Names for MYST1 Antibody (8C4C4)

• FLJ14040
• hMOFhistone acetyltransferase MYST1
• KAT8
• MOFEC 2.3.1.48
• MOZ, YBF2/SAS3, SAS2 and TIP60 protein 1
• MYST histone acetyltransferase 1
• MYST-1
• ortholog of Drosophila males absent on the first (MOF)
• probable histone acetyltransferase MYST1

Background

MYST1 (MYST histone acetyltransferase 1, MOF) belongs to the MYST family of histone acetyl transferases, which are employed in the cell to bring about transcriptional regulation. The MYST family includes MYST1, is named for the founding members MOZ, yeast YBF2 and SAS2, and TIP60. All members of this family contain a MYST region of about 240 amino acids with a canonical acetyl-CoA-binding site and a C2HC-type zinc finger motif. Most MYST proteins also have a chromodomain involved in protein- protein interactions and targeting transcriptional regulators to chromatin. Although MOF is expressed in both males and females, it associates with the X chromosome only in males. MOF contains a zinc-finger domain that is used to contact the globular part of the nucleosome and histone H4. The carboxy terminal domain of human MOF also has histone acetyl transferase activity directed against histones H3 and H2A, a characteristic shared with other MYST family histone.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: KAT8
• Entrez:
  • 84148(Human)
• Uniprot:
  • Q9H7Z6(Human)