Human Muscarinic Acetylcholine Receptor M3/CHRM3 ELISA Kit (Colorimetric)

ELISA: Human Muscarinic Acetylcholine Receptor M3/CHRM3 ELISA Kit (Colorimetric) [NBP2-81155] - Samples were spiked with high concentrations of Human Muscarinic Acetylcholine Receptor M3/CHRM3 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Human Muscarinic Acetylcholine Receptor M3/CHRM3 ELISA Kit (Colorimetric) [NBP2-81155] - Standard Curve Reference

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Serum, plasma, cell supernatant, and other biological fluids
• Standard Curve Range: 0.31 - 20 ng/mL
• Sensitivity: 0.19 ng/mL

Human Muscarinic Acetylcholine Receptor M3/CHRM3 ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes Human Muscarinic Acetylcholine Receptor M3/CHRM3 in samples. No significant cross-reactivity or interference between Human Muscarinic Acetylcholine Receptor M3/CHRM3 and analogues was observed.
• Standard Curve Range: 0.31 - 20 ng/mL
• Sensitivity: 0.19 ng/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: CV% < 4.84%
• Intra-Assay: CV% < 5.48%
• Spike Recovery: 86-102%
• Sample Volume: 100 ul
• Kit Type: ELISA Kit (Colorimetric)
• Gene: CHRM3

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human Muscarinic Acetylcholine Receptor M3/CHRM3 ELISA Kit (Colorimetric)

• cholinergic receptor, muscarinic 3
• CHRM3
• HM3
• m3 muscarinic receptor
• mAChR M3
• muscarinic acetylcholine receptor M3

Background

The muscarinic cholinergic receptors belong to a larger family of G protein-coupled receptors. The functional diversity of these receptors is defined by the binding of acetylcholine and includes cellular responses such as adenylate cyclase inhibition, phosphoinositide degeneration, and potassium channel mediation. Muscarinic receptors influence many effects of acetylcholine in the central and peripheral nervous system. The muscarinic cholinergic receptor 3 controls smooth muscle contraction and its stimulation causes secretion of glandular tissue.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for Muscarinic Acetylcholine Receptor M3/CHRM3 ELISA Kit (NBP2-81155)(1)

Publication using NBP2-81155

• Oğlak S, Aşır F, Korak T et al. Reduced M3 muscarinic acetylcholine receptor expression in gestational diabetes mellitus: implications for placental dysfunction and vascular regulation. The journal of maternal-fetal & neonatal medicine : the official journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians 2025-06-24 [PMID: 40545431]

FAQs for Muscarinic Acetylcholine Receptor M3/CHRM3 ELISA Kit (NBP2-81155). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: CHRM3