Human Muscarinic Acetylcholine Receptor M3/CHRM3 ELISA Kit (Colorimetric)

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ELISA: Human Muscarinic Acetylcholine Receptor M3/CHRM3 ELISA Kit (Colorimetric) [NBP2-81155] - Samples were spiked with high concentrations of Human Muscarinic Acetylcholine Receptor M3/CHRM3 and diluted with Reference ...read more
ELISA: Human Muscarinic Acetylcholine Receptor M3/CHRM3 ELISA Kit (Colorimetric) [NBP2-81155] - Standard Curve Reference

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma, cell supernatant, and other biological fluids
Standard Curve Range
0.31 - 20 ng/mL
Sensitivity
0.19 ng/mL

Order Details

Human Muscarinic Acetylcholine Receptor M3/CHRM3 ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Human Muscarinic Acetylcholine Receptor M3/CHRM3 in samples. No significant cross-reactivity or interference between Human Muscarinic Acetylcholine Receptor M3/CHRM3 and analogues was observed.
Standard Curve Range
0.31 - 20 ng/mL
Sensitivity
0.19 ng/mL
Assay Type
Sandwich-ELISA
Inter-Assay
CV% < 4.84%
Intra-Assay
CV% < 5.48%
Spike Recovery
86-102%
Sample Volume
100 ul
Kit Type
ELISA Kit (Colorimetric)
Gene
CHRM3

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25x)
  5. HRP Conjugate Diluent
  6. Micro ELISA Plate (Dismountable)
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Stop Solution
  12. Substrate Reagent

Alternate Names for Human Muscarinic Acetylcholine Receptor M3/CHRM3 ELISA Kit (Colorimetric)

  • cholinergic receptor, muscarinic 3
  • CHRM3
  • HM3
  • m3 muscarinic receptor
  • mAChR M3
  • muscarinic acetylcholine receptor M3

Background

The muscarinic cholinergic receptors belong to a larger family of G protein-coupled receptors. The functional diversity of these receptors is defined by the binding of acetylcholine and includes cellular responses such as adenylate cyclase inhibition, phosphoinositide degeneration, and potassium channel mediation. Muscarinic receptors influence many effects of acetylcholine in the central and peripheral nervous system. The muscarinic cholinergic receptor 3 controls smooth muscle contraction and its stimulation causes secretion of glandular tissue.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol CHRM3