Muscarinic Acetylcholine Receptor M3/CHRM3 Antibody

Western Blot: Muscarinic Acetylcholine Receptor M3/CHRM3 Antibody [NBP2-19444] - Whole cell extracts (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with mAChR M3 antibody at a dilution of 1:1000.

Immunocytochemistry/Immunofluorescence: Muscarinic Acetylcholine Receptor M3/CHRM3 Antibody [NBP2-19444] - A431 cells were fixed in ice-cold MeOH for 5 min. Green: mAChR M3 stained by mAChR M3 antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar= 10 um.

Immunocytochemistry/Immunofluorescence: Muscarinic Acetylcholine Receptor M3/CHRM3 Antibody [NBP2-19444] - A431 cells were fixed in ice-cold MeOH for 5 min. Green: mAChR M3 stained by mAChR M3 antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar= 10 um.

Product Discontinued

Muscarinic Acetylcholine Receptor M3/CHRM3 Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human Muscarinic acetylcholine receptor M3. The exact sequence is proprietary.
• Localization: Cell membrane; Cell junction; synapse; postsynaptic cell membrane; Basolateral cell membrane
• Predicted Species: Bovine (92%), Chimpanzee (100%), Rhesus Macaque (97%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CHRM3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Western Blot 1:500-1:3000
• Application Notes: Flow, IHC-FR -Assay dependent
• Theoretical MW: 66 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Porcine reactivity reported in scientific literature (PMID:33367142) Immunogen displays the following percentage of sequence identity for non-tested species: Rat (84%),Guinea Pig (84%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7), 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Immunogen affinity purified

Alternate Names for Muscarinic Acetylcholine Receptor M3/CHRM3 Antibody

• cholinergic receptor, muscarinic 3
• CHRM3
• HM3
• m3 muscarinic receptor
• mAChR M3
• muscarinic acetylcholine receptor M3

Background

The muscarinic cholinergic receptors belong to a larger family of G protein-coupled receptors. The functional diversity of these receptors is defined by the binding of acetylcholine and includes cellular responses such as adenylate cyclase inhibition, phosphoinositide degeneration, and potassium channel mediation. Muscarinic receptors influence many effects of acetylcholine in the central and peripheral nervous system. The muscarinic cholinergic receptor 3 controls smooth muscle contraction and its stimulation causes secretion of glandular tissue. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Muscarinic Acetylcholine Receptor M3/CHRM3 Antibody (NBP2-19444)(1)

Publication using NBP2-19444

• Jana B, Calka J, Palus K, Sikora M Escherichia coli -induced inflammation changes the expression of acetylcholine receptors (M2R, M3R, and alpha-7 nAChR) in the pig uterus Journal of Veterinary Research 2020-11-06 [PMID: 33367142] (WB, Porcine)

Bioinformatics

• Gene Symbol: CHRM3
• Uniprot:
  • P20309()