MT2A Antibody (1006811) [Unconjugated]

MT2A was detected in immersion fixed paraffin-embedded sections of human liver using Mouse Anti-Human MT2A Monoclonal Antibody (Catalog # MAB10176) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in hepatocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

• Reactivity: Hu
• Applications: IHC
• Clone: 1006811
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

MT2A Antibody (1006811) [Unconjugated] Summary

• Immunogen: Synthetic peptide containing human MT2A
  Accession # P02795
• Specificity: Detects human MT2A in diect ELISAs.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MT2A Antibody (1006811) [Unconjugated]

• MT2
• MT-2
• MT2A
• MT-II

Background

Metallothionein-2 (MT2A) is a protein that is transcriptionally regulated by heavy metals and glucocorticoids. This Metallothionein (MT) binds zinc. MTs sequester and/or dispense zinc during stress and inflammation to protect cells against reactive oxygen species.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • P02795()