MSH5 Antibody

Immunofluorescence: MSH5 Antibody [38750002] -This image is specific to animal number Q2374

Immunofluorescence: MSH5 Antibody [38750002] - This image is specific to animal number SDQ2376 1:10K Affinity Purified - Staining in N2

• Reactivity: Ce
• Applications: ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

MSH5 Antibody Summary

• Immunogen: This antibody was made against aa1164-1263 from the C Terminus Region of C. Elegans MSH5
• Specificity: This product is specific for C. elegans msh-5.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MSH5
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
• Application Notes: This antibody works in ELISA and Immunofluorescence. The concentration for this product is lot dependent. Check product vial for current lot concentration. Typically concentration ranges from 0.5 to 1.5 mg/ml. Use in Immunohistochemistry reported in scientific literature (PMID:23172739).

Reactivity Notes

C. elegans.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20mM Potassium Phosphate (pH 7.0) and 0.15M NaCl
• Preservative: No Preservative
• Purity: Immunogen affinity purified

Notes

This product was created from the ModEncode Project, a part of the NHGRI, and is sold by SDIX and Novus Biologicals. These C. elegans antibodies were generated in the labs of Jason Lieb, Susan Strome, Julie Ahringer, Arshad Desai, and Abby Dernburg.

Alternate Names for MSH5 Antibody

• DKFZp434C1615
• G7
• hMSH5
• MGC2939
• mutS (E. coli) homolog 5
• mutS homolog 5 (E. coli)
• mutS protein homolog 5
• MUTSH5
• NG23

Background

This gene encodes a member of the mutS family of proteins that are involved in DNA mismatch repair or meiotic recombination processes. This protein is similar to a Saccharomyces cerevisiae protein that participates in meiotic segregation fidelity and crossing-over. This protein forms heterooligomers with another member of this family, mutS homolog 4. Alternative splicing results in four transcript variants encoding three different isoforms.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MSH5 Antibody (38750002)(6)

Publications using 38750002

• Cahoon CK, Helm JM, Libuda DE Synaptonemal Complex Central Region Proteins Promote Localization of Pro-crossover Factors to Recombination Events During Caenorhabditis elegans Meiosis Genetics Aug 20 2019 [PMID: 31431470] (ICC/IF, C. elegans)
• Cahoon C, Helm J, Libuda D. Synaptonemal complex proteins direct and constrain the localization of crossover-promoting proteins during Caenorhabditis elegans meiosis bioRxiv Jan 18 2019 (ICC/IF, C. elegans)
• Woglar A, Villeneuve AM. Dynamic Architecture of DNA Repair Complexes and the Synaptonemal Complex at Sites of Meiotic Recombination. bioRxiv. [PMID: 29754818] (ICC/IF, C. elegans)
• Garrison C, Lastwika K, Zhang Y et al. Proteomic Analysis, Immune Dysregulation and Pathway Interconnections with Obesity. Journal of Proteome Research Oct 21 2016 [PMID: 27769113] (MiAr)
  
  Details:
  MSH5 Antibody used in microarray creation
• Rho JH, Lampe PD. High-throughput screening for native autoantigen-autoantibody complexes using antibody microarrays. Journal of Proteome Research May 3 2014 [PMID: 23541305] (MiAr)
  
  Details:
  Anti-MSH5 used in screening array
• Yajima M, Suglia E, Gustafson EA, Wessel GM. Meiotic gene expression initiates during larval development in the sea urchin Dev Dyn 2012 Nov 21 [PMID: 23172739] (IHC, ICC/IF, Invertebrate)

Bioinformatics

• Gene Symbol: MSH5
• Entrez:
  • 178268(C. elegans)
• Uniprot:
  • Q19272(C. elegans)