MRPL9 Antibody - BSA Free

Western Blot: MRPL9 Antibody [NBP3-06270] - Anti-MRPL9 rabbit polyclonal antibody at 1:500 dilutionLane A: HepG2 Whole Cell LysateLane B: RT-4 Whole Cell LysateLane C: U-251 MG Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L) HRP at 110000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:30 kDaObserved band size:28 kDa

Immunohistochemistry-Paraffin: MRPL9 Antibody [NBP3-06270] - Immunochemical staining of human MRPL9 in human small intestine with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.

Immunohistochemistry: MRPL9 Antibody [NBP3-06270] - Immunochemical staining of human MRPL9 in human brain with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.

Immunoprecipitation: MRPL9 Antibody [NBP3-06270] - MRPL9 was immunoprecipitated using:Lane A: 0.5 mg HepG2 Whole Cell Lysate4 uL anti-MRPL9 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein AG.Primary antibody:Anti-MRPL9 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Goat Anti-Rabbit IgG (H+L) HRP at 110000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 30 kDaObserved band size: 26 kDa

Immunocytochemistry/Immunofluorescence: MRPL9 Antibody [NBP3-06270] - Immunofluorescence staining of MRPL9 in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with NBP3-06270 antibody (dilution ratio 1:200) at 4 degrees celsius overnight. Then cells were stained with the Alexa Fluor (TM) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

MRPL9 Antibody - BSA Free Summary

• Description: This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
• Immunogen: Produced in rabbits immunized with E. coli-derived Human MRPL9 fragment.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MRPL9
• Purity: Antigen and protein A Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:500
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation 1-5uL/mg of lysate
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.03% Proclin 300
• Purity: Antigen and protein A Affinity-purified

Alternate Names for MRPL9 Antibody - BSA Free

• L9mt
• mitochondrial ribosomal protein L9
• MRP-L9,39S ribosomal protein L9, mitochondrial

Background

MRPL9, also known as Mitochondrial Ribosomal Protein L9, consists of a 267 amino acid isoform that is 30 kDa, and is involved in the manufacture of proteins within the mitochondria. Current disease research is working to determine the relationship between MRPL9 and obesity. MRPL9 has been linked to the process of translation, where it interacts with CA12, ICT1, SAT1, SLX4, and MRPL11.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MRPL9