MRPL47 Overexpression Lysate (Native)


Western Blot: MRPL47 Overexpression Lysate (Adult Normal) [NBL1-13274] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for MRPL47.

Product Details

Reactivity HuSpecies Glossary
Applications WB

Order Details

MRPL47 Overexpression Lysate (Native) Summary

The lysate was created in HEK293T cells, using plasmid ID RC207645 and based on accession number NM_177988. The protein contains a C-terminal DDK tag.
Homo sapiens mitochondrial ribosomal protein L47 (MRPL47), nuclear gene encoding mitochondrial protein, transcript variant 2, mRNA.


Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
16.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Store at -80C. Avoid freeze-thaw cycles.
RIPA buffer

Lysate Details for MRPL47

Protein State


HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for MRPL47 Overexpression Lysate (Native)

  • CGI-204
  • L47mt
  • MGC45403
  • mitochondrial ribosomal protein L47
  • MRP-L47
  • nasopharyngeal carcinoma metastasis-related 1
  • Nasopharyngeal carcinoma metastasis-related protein 1
  • NCM139S ribosomal protein L47, mitochondrial


Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. This gene is immediately adjacent to the gene for BAF complex 53 kDa subunit protein a (BAF53a), in a tail-to-tail orientation. Two transcript variants encoding different protein isoforms have been identified. Transcript Variant: This variant (2) lacks an alternate segment, compared to variant 1, which leads to the use of a downstream start codon. The predicted protein (isoform b) is shorter than isoform a. The predicted ORF of this transcript has not been experimentally confirmed.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Gene Symbol MRPL47