MON1A Antibody

Western Blot: MON1A Antibody [NBP1-52007] - Analysis of Mouse (A) and Rat (B) Testis lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Western Blot: MON1A Antibody [NBP1-52007] - MON1A in human Pancreas lysate (35ug protein in RIPA buffer) using NBP1-52007 at 1 ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu, Mu, Rt, Bv, Ca
• Applications: WB, ELISA, IP
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

MON1A Antibody Summary

• Description: Novus Biologicals Goat MON1A Antibody (NBP1-52007) is a polyclonal antibody validated for use in WB, ELISA and IP. Anti-MON1A Antibody: Cited in 4 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence HISYLEPDTDLC corresponding to internal region according to NP_001135973.1.
• Epitope: HISYLEPDTDLC
• Specificity: This antibody is expected to recognize isoforms a and b (NP_115731.2; NP_001135973.1). Amino acid numbering in name refers to sequence NP_115731.2.
• Predicted Species: Bovine (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: MON1A
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation
  • Peptide ELISA Detection limit 1:128000
  • Western Blot 1 - 3 ug/ml
• Application Notes: WB: Approx. 50 kDa band observed in human lung, pancreas and uterus lysates and in mouse and rat testis lysates (calculated MW of 55.5 kDa band according to NP_001135973.1). Use in IP reported in secitific publication PMID: 32543313

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for MON1A Antibody

• FLJ97088
• MON1 homolog A (yeast)
• SAND1MGC13272
• vacuolar fusion protein MON1 homolog A

Background

MON1A plays an important in membrane trafficking through the secretory apparatus. Not involved in endocytictrafficking to lysosomes

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MON1A Antibody (NBP1-52007)(4)

Publications using NBP1-52007

• Cai CZ, Zhuang XX, Zhu Q et al. Enhancing autophagy maturation with CCZ1-MON1A complex alleviates neuropathology and memory defects in Alzheimer disease models Theranostics 2022-01-24 [PMID: 35198070] (WB)
• Zeng H, Chen H, Li M et al. Autophagy protein NRBF2 attenuates endoplasmic reticulum stress-associated neuroinflammation and oxidative stress via promoting autophagosome maturation by interacting with Rab7 after SAH Journal of neuroinflammation 2021-09-16 [PMID: 34530854] (WB, Mouse)
• Cai CZ, Yang C, Zhuang XX et al. NRBF2 is a RAB7 effector required for autophagosome maturation and mediates the association of APP-CTFs with active form of RAB7 for degradation Autophagy 2020-06-16 [PMID: 32543313] (WB, IP, Mouse)
• Lim J, Hao T, Shaw C et al. A protein-protein interaction network for human inherited ataxias and disorders of Purkinje cell degeneration. Cell 2006-05-19 [PMID: 16713569]

Bioinformatics

• Gene Symbol: MON1A
• Entrez:
  • 84315(Human)
  • 72825(Mouse)
  • 315999(Rat)
• Uniprot:
  • NP_115731.2(Human)