MON1A Antibody

Western Blot: MON1A Antibody [NBP1-52007] - Analysis of Mouse (A) and Rat (B) Testis lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Western Blot: MON1A Antibody [NBP1-52007] - MON1A in human Pancreas lysate (35ug protein in RIPA buffer) using NBP1-52007 at 1 ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.

(A-B) N2S cells were transfected with Mon1a shRNA or nontargeting shRNA. The level GTP-RAB7 were detected by GST-R7BD affinity-isolation assay. Data are quantified as mean +/- SEM (n = 3). *P < 0.05, **P < 0.01, ***P < 0.001 vs. the relative control. (C-F) Cells were transfected with Mon1a shRNA, and the levels of SQSTM1 and LC3-II were examined by immunoblotting. *P < 0.05, **P < 0.01, ***P < 0.001 vs. the relative control. (G-I) Autophagosome maturation in N2a cells transfected with Mon1a shRNA or nontargeting shRNA were determined by the GFP-RFG-LC3 probe. Quantification data are presented as the mean +/- SEM, n = 20-25 cells from 3 independent experiments. *P < 0.05, **P < 0.01, vs. the relative control. Scale bar: 5 μm. (J-K) SQSTM1 levels were determined by immunofluorescence in control and Mon1a KD cells. Quantification data are presented as the mean +/- SEM, n = 20-25 cells from 3 independent experiments. *P < 0.05, **P < 0.01, vs. the relative control. Scale bar: 5 μm. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/35198070), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

(A-C) After the N2S cells were transfected with Flag-CCZ1/Flag-MON1A or Flag vehicle, the levels of FL-APP, APP-CTFs and Flag-CCZ1, Flag-MON1A were examined by immunoblotting. Data are quantified as mean +/- SEM (n = 3). *P < 0.05, **P < 0.01, vs. the relative control. (D-E) Intracellular and extracellular A beta 1-40 and A beta 1-42 levels in N2S cells over-expressing CCZ1-MON1A were determined by ELISA analysis. Data are quantified as mean +/- SEM (n = 3). *P < 0.05, **P < 0.01, ***P < 0.001 vs. the relative control. (F-H) N2S cells were transfected with Mon1a shRNA or nontargeting shRNA. The levels of FL-APP, APP-CTFs were detected by immunoblotting. Data are quantified as mean +/- SEM (n = 3). *P < 0.05, **P < 0.01, ***P < 0.001 vs. the relative control. (I-J) ELISA analysis of intracellular and extracellular A beta levels in control and Mon1a KD cells. Data are quantified as mean +/- SEM (n = 3). *P < 0.05, **P < 0.01, ***P < 0.001 vs. the relative control. (K-L) N2S cells over-expressing CCZ1-MON1A were transiently transfected with GFP-Lc3 and stained with APP antibody. The colocalization of GFP-LC3 and APP/APP-CTFs was visualized under confocal microscope. Quantification data are presented as the mean +/- SEM, n = 20-25 cells from 3 independent experiments. Scale bar, 7.5 μm. (M-N) N2S cells over-expressing CCZ1-MON1A were transiently transfected with mcherry-LAMP1 and the GFP-APP, and the colocalization of mcherry-LAMP1 and GFP-APP under basal or CQ treated conditions were visualized under confocal microscope. Quantification data were presented as the mean +/- SEM, n = 20-25 cells from 3 independent experiments. Scale bar, 7.5 μm. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/35198070), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

• Reactivity: Hu, Mu, Rt, Bv, Ca
• Applications: WB, ELISA, IP
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

MON1A Antibody Summary

• Immunogen: Peptide with sequence HISYLEPDTDLC corresponding to internal region according to NP_001135973.1.
• Epitope: HISYLEPDTDLC
• Specificity: This antibody is expected to recognize isoforms a and b (NP_115731.2; NP_001135973.1). Amino acid numbering in name refers to sequence NP_115731.2.
• Predicted Species: Bovine (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: MON1A
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation
  • Peptide ELISA Detection limit 1:128000
  • Western Blot 1 - 3 ug/ml
• Application Notes: WB: Approx. 50 kDa band observed in human lung, pancreas and uterus lysates and in mouse and rat testis lysates (calculated MW of 55.5 kDa band according to NP_001135973.1). Use in IP reported in secitific publication PMID: 32543313

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for MON1A Antibody

• FLJ97088
• MON1 homolog A (yeast)
• SAND1MGC13272
• vacuolar fusion protein MON1 homolog A

Background

MON1A plays an important in membrane trafficking through the secretory apparatus. Not involved in endocytictrafficking to lysosomes

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MON1A Antibody (NBP1-52007)(4)

Publications using NBP1-52007

• Cai CZ, Zhuang XX, Zhu Q et al. Enhancing autophagy maturation with CCZ1-MON1A complex alleviates neuropathology and memory defects in Alzheimer disease models Theranostics 2022-01-24 [PMID: 35198070] (WB)
• Zeng H, Chen H, Li M et al. Autophagy protein NRBF2 attenuates endoplasmic reticulum stress-associated neuroinflammation and oxidative stress via promoting autophagosome maturation by interacting with Rab7 after SAH Journal of neuroinflammation 2021-09-16 [PMID: 34530854] (WB, Mouse)
• Cai CZ, Yang C, Zhuang XX et al. NRBF2 is a RAB7 effector required for autophagosome maturation and mediates the association of APP-CTFs with active form of RAB7 for degradation Autophagy 2020-06-16 [PMID: 32543313] (WB, IP, Mouse)
• Lim J, Hao T, Shaw C et al. A protein-protein interaction network for human inherited ataxias and disorders of Purkinje cell degeneration. Cell 2006-05-19 [PMID: 16713569]

Bioinformatics

• Gene Symbol: MON1A
• Entrez:
  • 84315(Human)
  • 72825(Mouse)
  • 315999(Rat)
• Uniprot:
  • NP_115731.2(Human)