Product Search for mmp-1-antibody: Novus Biologicals

MAB901

MMP-1 Antibody (36665) [Unconjugated]

200 μg

MAB901

HAF007). A specific band was detected for MMP-1 at approximately 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

Western blot shows lysates of PC-3 human prostate cancer cell line. PVDF Membrane was probed with 2 µg/mL of Mouse Anti-Human MMP-1 Monoclonal Antibody (Catalog # MAB901) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # <a class=

HAF007). A specific band was detected for MMP-1 at approximately 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of PC-3 human prostate cancer cell line. PVDF Membrane was probed with 2 µg/mL of Mouse Anti-Human MMP-1 Monoclonal Antibody (Catalog # MAB901) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for MMP-1 at approximately 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

CTS003) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox">

MMP‑1 was detected in immersion fixed paraffin-embedded sections of human ovarian cancer tissue using 25 µg/mL Mouse Anti-Human MMP‑1 Monoclonal Antibody (Catalog # MAB901) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-AEC Cell & Tissue Staining Kit (red; Catalog # <a class=

CTS003) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

CTS002) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox">

MMP-1 was detected in immersion fixed paraffin-embedded sections of human ovarian array using Mouse Anti-Human MMP-1 Monoclonal Antibody (Catalog # MAB901) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # <a class=

CTS002) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

HAF018). A specific band was detected for MMP-1 at approximately 50 kDa (as indicated) in the parental PC-3 cell line, but is not detectable in knockout PC-3 cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

<b>Genetic Strategies Validation. </b>Western blot shows lysates of PC-3 human prostate cancer parental cell line and MMP-1 knockout PC-3 cell line (KO). PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human MMP-1 Monoclonal Antibody (Catalog # MAB901) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # <a class=

HAF018). A specific band was detected for MMP-1 at approximately 50 kDa (as indicated) in the parental PC-3 cell line, but is not detectable in knockout PC-3 cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

Inhibition of PKC alpha decreases the protein expression of MMP‐1 and MMP‐9 induced by cell cross‐talk in SMC. A, Evaluation of PKC alpha activation in control SMC (S NG) vs SMC previously co‐cultured with MAC in HG (Sc HG). Note that, the phosphorylated form of PKC alpha is significantly increased in SMC that were before exposed to MAC in HG conditions. Silencing of CCR2 or p65 decreases the phosphorylation of PKC alpha to control levels. B, Effect of PKC alpha inhibitor – Go 6976 on the MMP‐1 and MMP‐9 protein expression induced by SMC‐MAC cross‐talk. S NG – control SMC, Sc NG – SMC which were co‐cultured with MAC in normal glucose, Sc HG ‐ SMC which were co‐cultured with MAC in high glucose, Sc HGGo ‐ SMC which were co‐cultured with MAC in high glucose in presence of 1 μmol/L Go 6976. n = 3, *P < .05, **P < .01, #P < .05, ##P < .01 Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29992758), licensed under a CC-BY license. Not internally tested by R&D Systems.

<b>Biological Strategies Validation. </b>DDC up-regulates MMP-1 through ERK1/2 and Akt activation(A) Co-cultures were treated with 100 μM DDC for the indicated time. Phosphorylation of ERK1/2, p38 and Akt were determined by Western blotting analysis. The corresponding non-phosphorylated ERK1/2, p38, Akt and beta -actin were used for protein loading control. (B) Co-cultures were treated with or without 100 μM DDC for 24 h in the presence or absence of ERK1/2 inhibitor (U0126, 10 μM). MMP-1 protein levels were analysed by Western blotting. (C) Co-cultures were treated with or without 100 μM DDC for 24 h in the presence or absence of p38 inhibitor (SB203580, 10 μM). MMP-1 protein levels were analysed by Western blotting. (D) Co-cultures were treated with or without 100 μM DDC for 24 h in the presence or absence of Akt inhibitor (T3830, 50 μM). MMP-1 protein levels were analysed by Western blotting. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/23577625), licensed under a CC-BY license. Not internally tested by R&D Systems.

<b>Biological Strategies Validation. </b>DDC up-regulates MMP-1 expression in a dose-dependent manner and lowered collagen I in LX-2 cellsCo-culture systems were treated with 25, 50, 100 and 200 μM DDC for 24 h. LX-2 cells and the culture medium were collected and analysed. (A) Quantitative RT-PCR results of MMP-1 expression in LX-2 cells. The results were normalized with beta -actin and expressed as fold increase relative to the control in each experiment. (B) 20 μg aliquots of total protein extracts from LX-2 cells were subjected to Western blotting analysis with anti-MMP-1 antibody (MAB 901 and MAB 3223 for active form only) and beta -actin antibody as a loading control, as described in the Materials and Methods section. (C) 30 μg of LX-2 lysates or 20 μl aliquots of 10-fold concentrated culture medium with 5×loading buffer were assayed by Casein Zymography for MMP-1 activity. (D) Levels of collagen I in LX-2 cells were assayed by western blotting. (E) Levels of collagen I in the culture medium were assayed by ELISA. *P<0.05 compared with untreated C3A-2E1/LX-2. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/23577625), licensed under a CC-BY license. Not internally tested by R&D Systems.

Co‐culture of SMC and MAC in high glucose conditions (24 h) induces a significant increase in the protein expression of MMP‐1 and MMP‐9. A, B: Protein expression of MMP‐1 (A) and MMP‐9 (B) was assessed in SMC after co‐culture with THP‐derived macrophages and evaluated by Western blot assay. C, D: Protein expression of MMP‐1 (C) and MMP‐9 (D) in SMC after co‐culture with human macrophages derived from freshly isolated monocytes from control subjects (CM) or diabetic patients with ACS (DM). S NG –control SMC, S HG – SMC exposed to HG, Sc NG or Sc CM – SMC which were co‐cultured with MAC in normal glucose, Sc HG or Sc DM ‐ SMC which were co‐cultured with MAC in high glucose. n = 3, *P < .05, **P < .01, ***P < .001, #P < .05, ##P < .01, ###P < .001 Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29992758), licensed under a CC-BY license. Not internally tested by R&D Systems.

Effect of CCR2 and p65 silencing in the regulation of MMP‐1 and MMP‐9 expression in SMC. Protein expression of MMP‐1 (A) and MMP‐9 (B) determined by Western blot in control SMC (S NG), SMC which were co‐cultured with MAC in HG conditions (Sc HG), and SMC subjected to silencing of CCR‐2 and p65 and co‐cultured with MAC in HG conditions (Cneg, p65, CCR2 siRNA). Note that silencing of CCR2 or p65 decreases significantly the protein expression of MMP‐1 and MMP‐9 induced by SMC‐MAC co‐culture in high glucose conditions. n = 3, *P < .05, #P < .05 Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29992758), licensed under a CC-BY license. Not internally tested by R&D Systems.

<b>Biological Strategies Validation. </b>DDC up-regulates MMP-1 expression in an H2O2-associated mannerCo-cultures were treated with 100 μM DDC, 2000 Units catalase, 25 μM vitamin E and 5μM H2O2 combined with 100 μM DDC for 24 h. Cell lysates from the LX-2 cells were collected and analysed. (A) 20 μg aliquots of total protein extracts from LX-2 cells were subjected to Western blotting analysis with anti-MMP-1 antibody (MAB 901) and beta -actin antibody as a loading control, as described in the Materials and Methods section. (B) Levels of collagen I in LX-2 cells were assayed by Western blotting. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/23577625), licensed under a CC-BY license. Not internally tested by R&D Systems.

<b>Biological Strategies Validation. </b>DDC up-regulates MMP-1 expression in LX-2 cellsCo-culture systems were treated with 100 μM DDC for 24 h. LX-2 cells and the culture medium were collected and analysed. (A) Quantitative RT-PCR results of MMP-1 expression in LX-2 cells. The results were normalized with beta -actin and expressed as fold increase relative to the control in each experiment. (B) 20 μg aliquots of total protein extracts from LX-2 cells were subjected to western blotting analysis with anti-MMP-1 antibody (MAB 901) and beta -actin antibody as a loading control, as described in the Materials and Methods section. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/23577625), licensed under a CC-BY license. Not internally tested by R&D Systems.

MMP-1, MMP-3, and MMP-13 expression. (A-D) MMP-1; (E-H) MMP-3; (I-L) MMP-13 (A,E,I) ACL from young normal knee; (B,F,J) ACL from aging knee; (C,G,K) fibroblast-like cell aggregates in the degenerated ACL; (D,H,L) chondrocyte-like cell aggregates in the degenerated ACL. (Original magnification ×40). The graph (M) represents the percentage (mean ± SEM) of MMP-1-, MMP-3-, and MMP-13-positive cells in each group. **P < 0.01. (N) Western blotting for MMP-1, MMP-3, and MMP-13 (n = 3 for each category). Image collected and cropped by CiteAb from the following open publication (//arthritis-research.biomedcentral.com/articles/10.1186/ar4165), licensed under a CC-BY license. Not internally tested by R&D Systems.

AEG-1 knockdown down-regulated MMP1 expression in HNSCC cells in vitro and in vivo. (A) microarray analysis of gene expression after AEG-1 knockdown in SAS cells. Genes with an absolute fold change greater than 2.5 are shown. SNORD3B-1, Homo sapiens small nucleolar RNA, C/D box 3B-1; EMILIN2, elastin microfibril interfacer 2; FABP4, fatty acid binding protein 4; MMP1, matrix metallopeptidase 1; ANO1, anoctamin 1; RELN, reelin; FPR2, formyl peptide receptor 2; UHMK1, U2AF homology motif kinase 1; RMND5A, required for meiotic nuclear division 5 homolog A; CAPZA1; capping protein (actin filament) muscle Z-line, alpha 1. *, p < 0.05; **, p < 0.01; ***, p < 0.001. (B) RT-QPCR confirmation of gene expression profiles after knockdown of AEG-1 in SAS and FaDu cells. All experiments were performed in triplicate (n = 3) and data were normalized to GAPDH. (C) secreted MMP1 protein in cell-conditioned culture media. Each cell lines were seeded in equal numbers (1 × 106 cells) and were cultured in starving condition (1% fetal bovine serum, FBS) for 24 hr and the media were harvested. (D) immunohistochemical staining of MMP1 in murine subcutaneous xenograft tumors (upper row) and metastatic lesions from in vivo lung metastasis assays (lower row). Scale bar: upper row, 100 μm; lower row, 25 μm. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/24063540), licensed under a CC-BY license. Not internally tested by R&D Systems.

Expression of MMP1 on gene and protein levels upon treatment with mitomycin C (MMC) of SKOV3 cells in normoxia and hypoxia. MMP1 gene expression was measured using (A) HTA 2.0 microarrays and (B) RT-qPCR [normalized to PPIA and GUSB levels and normoxia control (NC) as 1]. (C) Immunoblot of MMP 1 protein (with vinculin as loading control). (D) Quantification of MMP1 protein levels by densitometry. (E) Secretion of pro-MMP1 protein levels measured by ELISA.(F) Secretion of total MMP1 protein levels measured by ELISA. (B,D) Results were normalized to normoxia control as 1. ****p < 0.0001, ***p < 0.001, **p < 0.01, and *p < 0.05; VIN, vinculin. A detailed statistics description is provided in the Supplementary Table S2. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/40584967), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , , ,

Applications: , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB901U-100UG
Alexa Fluor 405	FAB901V-100UG
Alexa Fluor 488	FAB901G-100UG
Alexa Fluor 532	FAB901X-100UG
Alexa Fluor 594	FAB901T-100UG
Alexa Fluor 647	FAB901R-100UG
Alexa Fluor 700	FAB901N-100UG
Alexa Fluor 750	FAB901S-100UG
Alexa Fluor Plus 405	FAB901AFP405-100UG
Alexa Fluor Plus 488	FAB901AFP488-100UG
Alexa Fluor Plus 555	FAB901AFP555-100UG
Alexa Fluor Plus 594	FAB901AFP594-100UG
Alexa Fluor Plus 647	FAB901AFP647-100UG
Alexa Fluor Plus 680	FAB901AFP680-100UG

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB901-SP

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NBP2-67415

MMP-14/MT1-MMP Antibody (3-F7)

(1 Review)

(2 Publications)

100 ul

NBP2-67415

Immunohistochemistry-Paraffin: MMP-14/MT1-MMP Antibody (3-F7) [NBP2-67415] - Human endometrial adenocarcinoma paraffin-embedded tissue section. Tissue section was fixed, deparaffinized and EDTA was used for antigen retrieval. Incubated for 10 hours with primary antibody. IHC-P image submitted by a verified customer review.

Flow Cytometry: MMP-14/MT1-MMP Antibody (3-F7) [NBP2-67415] - Analysis of A549 cells with MMP14 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

Immunocytochemistry/Immunofluorescence: MMP-14/MT1-MMP Antibody (3-F7) [NBP2-67415] - Staining MMP14 in CRC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: MMP-14/MT1-MMP Antibody (3-F7) [NBP2-67415] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-MMP14 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: MMP-14/MT1-MMP Antibody (3-F7) [NBP2-67415] - Analysis of paraffin-embedded human kidney tissue using anti-MMP14 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: MMP-14/MT1-MMP Antibody (3-F7) [NBP2-67415] - Analysis of paraffin-embedded human uterus tissue using anti-MMP14 antibody. Counter stained with hematoxylin.

Western blot analysis on different lysates with NBP2-67415 at 1/1,000 dilution. Lane 1: NIH/3T3 cell lysate Lane 2: MEF cell lysate Lane 3: L6 cell lysate Lane 4: Mouse lung tissue lysate Lane 5: Rat lung tissue lysate Lysates/proteins at 20/40 µg/Lane. Predicted band size: 66 kDa Observed band size: 55 kDa Exposure time: 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP2-67415 at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

Western blot analysis on different lysates with NBP2-67415 at 1/1,000 dilution. Lane 1: human kidney Lysates/proteins at 20 µg/Lane. Exposure time: 30 seconds Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: NBP2-67415, 1/1,000 in primary antibody dilution buffer, overnight at 4 ℃ Secondary antibody: Goat Anti-Rabbit IgG [HRP] Secondary Antibody, 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 66 kDa Observed band size: 55 kDa

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , , ,

Applications: , , , ,

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

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MAB9071

MMP-7 Antibody (111433) [Unconjugated]

(18 Publications)

100 μg

MAB9071

WBC016). PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human MMP-7 Monoclonal Antibody (Catalog # MAB9071) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for MMP-7 at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

Western blot shows lysates of Capan-1 human pancreatic adenocarcinoma cell line and SK-OV-3 human ovarian adenocarcinoma cell line and, for additional reference, Recombinant Human MMP-7 Western Blot Standard Protein (Catalog # <a class=

WBC016). PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human MMP-7 Monoclonal Antibody (Catalog # MAB9071) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for MMP-7 at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of Capan-1 human pancreatic adenocarcinoma cell line and SK-OV-3 human ovarian adenocarcinoma cell line and, for additional reference, Recombinant Human MMP-7 Western Blot Standard Protein (Catalog # WBC016). PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human MMP-7 Monoclonal Antibody (Catalog # MAB9071) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for MMP-7 at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

Simple Western lane view shows lysates of SK‑OV‑3 human ovarian adenocarcinoma cell line, human small intestine tissue, and CaSki human cervical carcinoma, loaded at 0.5 mg/mL. Specific bands were detected for MMP‑7 at approximately 49 and 65 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human MMP-7 Monoclonal Antibody (Catalog # MAB9071) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

<b>Genetic Strategies Validation. </b>Zymography analysis for active MMP2 was performed with 4 μg of total protein of the conditioned medium of U87 (a) and primary IC cells (b) after 48 h exposure to JS-K (1–3 μM) on gelatin-containing PAGE. Western blot analysis for MMP2, MMP7, MMP9 and TIMP3 was performed for conditioned medium of U87 (a) and IC (b) cells with 4 μg of total protein as well as of 20 μg of total cell lysate of U87 (c) and IC cells (d) on SDS-PAGE. Conditioned media of knockdown and overexpression of ATF3 were examined for active MMP2 as well as the plasmid controls and uninfected U87 (e). 4 μg of protein of the supernatant (e) and 20 μg of cell lysates (f) were screened for MMP2, MMP7, MMP9 and TIMP3 by SDS-PAGE. Coomassie staining of the gels was used as a loading control for zymogram and for western blot of the supernatant, GAPDH was used as a loading control for cell lysates. Figures are representative for three independent experiments. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28250971), licensed under a CC-BY license. Not internally tested by R&D Systems.

<b>Genetic Strategies Validation. </b>Western blot shows culture media collected from A549 human lung carcinoma parental cell line and MMP‑7 knockout A549 cell line (KO). Nitrocellulose membrane was probed with Mouse Anti-Human MMP‑7 Monoclonal Antibody (Catalog # MAB9071) followed by HRP-conjugated secondary antibody. A specific band was detected for MMP‑7 at approximately 29.7 kDa (as indicated) in the culture media collected from parental A549 cell line, but is not detectable in the culture media collected from knockout A549 cell line. Primary antibody dilution used: 1 μg/ml. The Ponceau stained transfer of the blot is shown. This experiment was conducted under reducing conditions. Image, protocol, and testing courtesy of YCharOS Inc. See ycharos.com for additional details.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB9071U-100UG
Alexa Fluor 405	FAB9071V-100UG
Alexa Fluor 488	FAB9071G-100UG
Alexa Fluor 532	FAB9071X-100UG
Alexa Fluor 594	FAB9071T-100UG
Alexa Fluor 647	FAB9071R-100UG
Alexa Fluor 700	FAB9071N-100UG
Alexa Fluor 750	FAB9071S-100UG
Alexa Fluor Plus 405	FAB9071AFP405-100UG
Alexa Fluor Plus 488	FAB9071AFP488-100UG
Alexa Fluor Plus 555	FAB9071AFP555-100UG
Alexa Fluor Plus 594	FAB9071AFP594-100UG
Alexa Fluor Plus 647	FAB9071AFP647-100UG
Alexa Fluor Plus 680	FAB9071AFP680-100UG
Phycoerythrin	IC9071P

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	IC9071P
Carrier Free	MAB9071-SP
Carrier Free	MAB9071-500

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AF513

MMP-3 Antibody [Unconjugated]

(11 Publications)

100 μg

AF513

WBC015) and lysate of U-118-MG human glioblastoma/astrocytoma cell line. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human/Primate MMP-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF513) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for MMP-3 at approximately 57-60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

Western blot shows Recombinant Human MMP-3 Western Blot Standard Protein (<a class=

WBC015) and lysate of U-118-MG human glioblastoma/astrocytoma cell line. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human/Primate MMP-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF513) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for MMP-3 at approximately 57-60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows Recombinant Human MMP-3 Western Blot Standard Protein (WBC015) and lysate of U-118-MG human glioblastoma/astrocytoma cell line. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human/Primate MMP-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF513) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for MMP-3 at approximately 57-60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox">

MMP-3 was detected in immersion fixed paraffin-embedded sections of human bladder cancer tissue using Goat Anti-Human/ Primate MMP-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF513) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; <a class=

CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox">

MMP-3 was detected in immersion fixed paraffin-embedded sections of human lung cancer using Goat Anti-Human/Primate MMP-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF513) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; <a class=

CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

513-MP) was serially diluted and captured by Goat Anti-Human/Primate MMP‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF513) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human/Primate MMP‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF513) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox">

Recombinant Human MMP‑3 (Catalog # <a class=

513-MP) was serially diluted and captured by Goat Anti-Human/Primate MMP‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF513) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human/Primate MMP‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF513) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

513-MP) was serially diluted and captured by Goat Anti-Human/Primate MMP‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF513) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human/Primate MMP‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF513) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox">

513-MP) was serially diluted and captured by Goat Anti-Human/Primate MMP‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF513) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human/Primate MMP‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF513) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

Reactivity:

Hu, Pm

Species Glossary

Reactivity: Hu, Pm

Applications:

, , ,

Applications: , , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF513U-100UG
Alexa Fluor 405	AF513V-100UG
Alexa Fluor 488	AF513G-100UG
Alexa Fluor 532	AF513X-100UG
Alexa Fluor 594	AF513T-100UG
Alexa Fluor 647	AF513R-100UG
Alexa Fluor 700	AF513N-100UG
Alexa Fluor 750	AF513S-100UG
Alexa Fluor Plus 405	AF513AFP405-100UG
Alexa Fluor Plus 488	AF513AFP488-100UG
Alexa Fluor Plus 555	AF513AFP555-100UG
Alexa Fluor Plus 594	AF513AFP594-100UG
Alexa Fluor Plus 647	AF513AFP647-100UG
Alexa Fluor Plus 680	AF513AFP680-100UG
Biotin	BAF513

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF513-SP

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AF901

MMP-1 Antibody [Unconjugated]

(10 Publications)

100 μg

AF901

HAF017). A specific band was detected for MMP-1 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

Western blot shows lysates of PC-3 human prostate cancer cell line and HEK001 human epidermal keratinocyte cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human MMP-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF901) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=

HAF017). A specific band was detected for MMP-1 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of PC-3 human prostate cancer cell line and HEK001 human epidermal keratinocyte cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human MMP-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF901) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for MMP-1 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

<b>Left:</b> Simple Western lane view shows lysates of MDA‑MB‑231 human breast cancer cell line, loaded at 0.1 mg/ml. A specific band was detected for MMP‑1 at approximately 55 kDa (as indicated) using both 10 µg/ml and 50 µg/ml of Goat Anti-Human MMP‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF901) followed by HRP-conjugated Donkey Anti-Goat Secondary Antibody (Catalog # 043-522-2). This experiment was conducted under reducing conditions and using the 12-230kDa separation system. <b>Right:</b> Simple Western electropherogram showing the same Goat Anti-Human MMP‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF901) tested at 10 µg/ml (blue line) and 50 µg/ml (green line) in the MDA‑MB‑231 human breast cancer cell line.

CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox">

MMP-1 was detected in immersion fixed paraffin-embedded sections of human ovarian cancer tissue using 15 µg/mL Goat Anti-Human MMP-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF901) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # <a class=

CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox">

MMP-1 was detected in immersion fixed paraffin-embedded sections of human prostate tissue using Goat Anti-Human MMP-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF901) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # <a class=

CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

HAF017). A specific band was detected for MMP-1 at approximately 50 kDa (as indicated) in the parental PC-3 cell line, but is not detectable in knockout PC-3 cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

HAF017). A specific band was detected for MMP-1 at approximately 50 kDa (as indicated) in the parental PC-3 cell line, but is not detectable in knockout PC-3 cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , , ,

Applications: , , , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF901U-100UG
Alexa Fluor 405	AF901V-100UG
Alexa Fluor 488	AF901G-100UG
Alexa Fluor 532	AF901X-100UG
Alexa Fluor 594	AF901T-100UG
Alexa Fluor 647	AF901R-100UG
Alexa Fluor 700	AF901N-100UG
Alexa Fluor 750	AF901S-100UG
Alexa Fluor Plus 405	AF901AFP405-100UG
Alexa Fluor Plus 488	AF901AFP488-100UG
Alexa Fluor Plus 555	AF901AFP555-100UG
Alexa Fluor Plus 594	AF901AFP594-100UG
Alexa Fluor Plus 647	AF901AFP647-100UG
Alexa Fluor Plus 680	AF901AFP680-100UG
Biotin	BAF901

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF901-SP

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NB100-91878

MMP-3 Antibody - BSA Free

(12 Publications)

0.1 ml

NB100-91878

Immunohistochemistry-Paraffin: MMP-3 Antibody [NB100-91878] - Analyzes of paraffin-embedded human breast carcinoma tissue.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

,

Applications: ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NB100-91878AF350
Alexa Fluor 405	NB100-91878AF405
Alexa Fluor 488	NB100-91878AF488
Alexa Fluor 532	NB100-91878AF532
Alexa Fluor 594	NB100-91878AF594
Alexa Fluor 647	NB100-91878AF647
Alexa Fluor 700	NB100-91878AF700
Alexa Fluor 750	NB100-91878AF750
Alexa Fluor Plus 405	NB100-91878AFP405
Alexa Fluor Plus 488	NB100-91878AFP488
Alexa Fluor Plus 555	NB100-91878AFP555
Alexa Fluor Plus 594	NB100-91878AFP594
Alexa Fluor Plus 647	NB100-91878AFP647
Alexa Fluor Plus 680	NB100-91878AFP680
Allophycocyanin	NB100-91878APC
Biotin	NB100-91878B
CoraFluor 1	NB100-91878CL1
DyLight 350	NB100-91878UV
DyLight 405	NB100-91878V
DyLight 488	NB100-91878G
DyLight 550	NB100-91878R
DyLight 594	NB100-91878DL594
DyLight 650	NB100-91878C
DyLight 680	NB100-91878FR
DyLight 755	NB100-91878IR
FITC	NB100-91878F
HRP	NB100-91878H
Janelia Fluor 525	NB100-91878JF525
Janelia Fluor 549	NB100-91878JF549
Janelia Fluor 646	NB100-91878JF646
Janelia Fluor 669	NB100-91878JF669
PE	NB100-91878PE
PerCP	NB100-91878PCP
mFluor Violet 450 SE	NB100-91878MFV450
mFluor Violet 500 SE	NB100-91878MFV500
mFluor Violet 610 SE	NB100-91878MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NB100-91878SS

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MAB9181

MMP-14/MT1-MMP Antibody (128527) [Unconjugated]

(11 Publications)

100 μg

MAB9181

MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B)." class="big_lightbox">

MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B)." alt="MDA-MB-231 human breast cancer cell line was stained with Mouse Anti-Human MMP-14/MT1-MMP Monoclonal Antibody (Catalog # MAB9181, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B)." class="big_thumb" />

VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in epithelial cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox">

MMP‑14/MT1‑MMP was detected in immersion fixed paraffin-embedded sections of Human Prostate Cancer using Mouse Anti-Human MMP‑14/MT1‑MMP Monoclonal Antibody (Catalog # MAB9181) at 15 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # <a class=

VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in epithelial cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , , ,

Applications: , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB9181U-100UG
Alexa Fluor 405	FAB9181V-100UG
Alexa Fluor 488	FAB9181G
Alexa Fluor 594	FAB9181T-100UG
Alexa Fluor 647	FAB9181R-100UG
Alexa Fluor 700	FAB9181N-100UG
Alexa Fluor 750	FAB9181S-100UG
Alexa Fluor Plus 405	FAB9181AFP405-100UG
Alexa Fluor Plus 488	FAB9181AFP488-100UG
Alexa Fluor Plus 555	FAB9181AFP555-100UG
Alexa Fluor Plus 594	FAB9181AFP594-100UG
Alexa Fluor Plus 647	FAB9181AFP647-100UG
Alexa Fluor Plus 680	FAB9181AFP680-100UG
Allophycocyanin	FAB9181A
Phycoerythrin	FAB9181P

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	FAB9181A
Carrier Free	FAB9181G
Carrier Free	FAB9181P
Carrier Free	MAB9181-SP
Carrier Free	MAB9181-500
Carrier Free	FAB9181V-100UG
Carrier Free	FAB9181U-100UG
Carrier Free	FAB9181T-100UG
Carrier Free	FAB9181S-100UG
Carrier Free	FAB9181R-100UG
Carrier Free	FAB9181N-100UG

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AF907

MMP-7 Antibody [Unconjugated]

(8 Publications)

100 μg

AF907

HAF017). A specific band was detected for MMP-7 at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

Western blot shows lysates of Capan-1 human pancreatic adenocarcinoma cell line and HT-29 human colon adenocarcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human MMP-7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF907) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (<a class=

HAF017). A specific band was detected for MMP-7 at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of Capan-1 human pancreatic adenocarcinoma cell line and HT-29 human colon adenocarcinoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human MMP-7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF907) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for MMP-7 at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox">

MMP-7 was detected in immersion fixed paraffin-embedded sections of human pancreas array using Goat Anti-Human MMP-7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF907) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # <a class=

CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_lightbox">

Simple Western lane view shows lysates of normal stem cells (negative control) and Crohn's stem cells, loaded at 0.2 mg/mL. A specific band was detected for MMP-7 at approximately 10 kDa (as indicated) using 10 µg/mL of Goat Anti-Human MMP-7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF907) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (<a class=

HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_thumb" />

ES001) by Recombinant Human MMP-7 (0.2 µg/mL, Catalog # 907-MP) is measured after preincubation with increasing concentrations of Goat Anti-Human MMP-7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF907). The ND₅₀ is typically 2 µg/mL." class="big_lightbox">

The cleavage of Mca-PLGL-Dpa-AR-NH<SUB>2</SUB>(10 µM, Catalog # <a class=

ES001) by Recombinant Human MMP-7 (0.2 µg/mL, Catalog # 907-MP) is measured after preincubation with increasing concentrations of Goat Anti-Human MMP-7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF907). The ND₅₀ is typically 2 µg/mL." class="big_thumb" />

AF907) at 3ug/mL with overnight incubation at 4 degrees Celsius followed by incubation with anti-goat IgG VisUCyte HRP Polymer Antibody (Catalog # VC004) and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). Specific staining was localized to exocrine glands. " class="big_lightbox">

Formalin-fixed paraffin-embedded tissue sections of human pancreas were probed for MMP7 mRNA (ACD RNAScope Probe, catalog #488408; Fast Red chromogen, ACD catalog # 322750). Adjacent tissue section was processed for immunohistochemistry using goat anti-human MMP7 polyclonal antibody (R&D Systems catalog # <a class=

AF907) at 3ug/mL with overnight incubation at 4 degrees Celsius followed by incubation with anti-goat IgG VisUCyte HRP Polymer Antibody (Catalog # VC004) and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue). Specific staining was localized to exocrine glands. " class="big_thumb" />

907-MP) was serially diluted and captured by Mouse Anti-Human/Primate MMP‑7 Monoclonal Antibody (Catalog # MAB9072) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human MMP‑7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF907) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox">

Recombinant Human MMP‑7 (Catalog # <a class=

907-MP) was serially diluted and captured by Mouse Anti-Human/Primate MMP‑7 Monoclonal Antibody (Catalog # MAB9072) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human MMP‑7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF907) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , , , ,

Applications: , , , , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF907U-100UG
Alexa Fluor 405	AF907V-100UG
Alexa Fluor 488	AF907G-100UG
Alexa Fluor 532	AF907X-100UG
Alexa Fluor 594	AF907T-100UG
Alexa Fluor 647	AF907R-100UG
Alexa Fluor 700	AF907N-100UG
Alexa Fluor 750	AF907S-100UG
Alexa Fluor Plus 405	AF907AFP405-100UG
Alexa Fluor Plus 488	AF907AFP488-100UG
Alexa Fluor Plus 555	AF907AFP555-100UG
Alexa Fluor Plus 594	AF907AFP594-100UG
Alexa Fluor Plus 647	AF907AFP647-100UG
Alexa Fluor Plus 680	AF907AFP680-100UG
Biotin	BAF907

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF907-SP

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NBP2-22123

MMP-1 Antibody (6A5) - BSA Free

(5 Publications)

0.1 ml

NBP2-22123

Immunohistochemistry-Paraffin: MMP-1 Antibody (6A5) [NBP2-22123] - Immunohistochemical analysis of paraffin-embedded human cervical cancer tissues (left) and human kidney cancer tissues (right) using MMP1 mouse mAb with DAB staining.

Flow Cytometry: MMP-1 Antibody (6A5) [NBP2-22123] - Flow cytometric analysis of Hela cells using MMP1 mouse mAb (green) and negative control (purple).

ELISA: MMP-1 Antibody (6A5) [NBP2-22123] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng).

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

, , , ,

Applications: , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-22123AF350
Alexa Fluor 405	NBP2-22123AF405
Alexa Fluor 488	NBP2-22123AF488
Alexa Fluor 532	NBP2-22123AF532
Alexa Fluor 594	NBP2-22123AF594
Alexa Fluor 647	NBP2-22123AF647
Alexa Fluor 700	NBP2-22123AF700
Alexa Fluor 750	NBP2-22123AF750
Alexa Fluor Plus 405	NBP2-22123AFP405
Alexa Fluor Plus 488	NBP2-22123AFP488
Alexa Fluor Plus 555	NBP2-22123AFP555
Alexa Fluor Plus 594	NBP2-22123AFP594
Alexa Fluor Plus 647	NBP2-22123AFP647
Alexa Fluor Plus 680	NBP2-22123AFP680
Allophycocyanin	NBP2-22123APC
Allophycocyanin/Cy7	NBP2-22123APCCY7
Biotin	NBP2-22123B
CoraFluor 1	NBP2-22123CL1
DyLight 350	NBP2-22123UV
DyLight 405	NBP2-22123V
DyLight 488	NBP2-22123G
DyLight 550	NBP2-22123R
DyLight 594	NBP2-22123DL594
DyLight 650	NBP2-22123C
DyLight 680	NBP2-22123FR
DyLight 755	NBP2-22123IR
FITC	NBP2-22123F
HRP	NBP2-22123H
Janelia Fluor 525	NBP2-22123JF525
Janelia Fluor 549	NBP2-22123JF549
Janelia Fluor 646	NBP2-22123JF646
Janelia Fluor 669	NBP2-22123JF669
PE	NBP2-22123PE
PE/Atto594	NBP2-22123PEATT594
PE/Cy5.5	NBP2-22123PECY55
PE/Cy7	NBP2-22123PECY7
PerCP	NBP2-22123PCP
mFluor Violet 450 SE	NBP2-22123MFV450
mFluor Violet 500 SE	NBP2-22123MFV500
mFluor Violet 610 SE	NBP2-22123MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP2-22123SS
Azide and BSA Free	NBP2-80852

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MAB513

MMP-3 Antibody (50647) [Unconjugated]

(1 Review)

(4 Publications)

500 μg

MAB513

WBC015). PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human MMP-3 Monoclonal Antibody (Catalog # MAB513) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for MMP-3 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

Western blot shows Recombinant Human MMP-3 Westrern Blot Standard Protein (2 µL, Catalog # <a class=

WBC015). PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human MMP-3 Monoclonal Antibody (Catalog # MAB513) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for MMP-3 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows Recombinant Human MMP-3 Westrern Blot Standard Protein (2 µL, Catalog # WBC015). PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human MMP-3 Monoclonal Antibody (Catalog # MAB513) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for MMP-3 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in cancer cells (right panel). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox">

MMP-3 was detected in immersion fixed paraffin-embedded sections of normal human prostate and human prostate cancer tissue using Mouse Anti-Human MMP-3 Monoclonal Antibody (Catalog # MAB513) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # <a class=

CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in cancer cells (right panel). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox">

MMP-3 was detected in immersion fixed MG-63 human osteosarcoma cell line using 10 µg/mL Mouse Anti-Human MMP-3 Monoclonal Antibody (Catalog # MAB513) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # <a class=

NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules." class="big_lightbox">

MG-63 human osteosarcoma cell line was stained with Mouse Anti-Human MMP-3 Monoclonal Antibody (Catalog # MAB513, filled histogram) or isotype control antibody (Catalog # <a class=

MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules." class="big_thumb" />

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , , ,

Applications: , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	IC513U-100UG
Alexa Fluor 405	IC513V-100UG
Alexa Fluor 488	IC513G-100UG
Alexa Fluor 594	IC513T-100UG
Alexa Fluor 647	IC513R-100UG
Alexa Fluor 700	IC513N-100UG
Alexa Fluor 750	IC513S-100UG
Alexa Fluor Plus 405	FAB513AFP405-100UG
Alexa Fluor Plus 488	FAB513AFP488-100UG
Alexa Fluor Plus 555	FAB513AFP555-100UG
Alexa Fluor Plus 594	FAB513AFP594-100UG
Alexa Fluor Plus 647	FAB513AFP647-100UG
Alexa Fluor Plus 680	FAB513AFP680-100UG

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB513-SP
Carrier Free	IC513G-100UG
Carrier Free	IC513V-100UG
Carrier Free	IC513U-100UG
Carrier Free	IC513T-100UG
Carrier Free	IC513S-100UG
Carrier Free	IC513R-100UG
Carrier Free	IC513N-100UG

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MAB918

MMP-14/MT1-MMP Antibody (5H2) [Unconjugated]

(1 Review)

(3 Publications)

500 μg

MAB918

HAF018). Specific bands were detected for MMP-14/MT1-MMP at approximately 50 kDa and 62 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

Western blot shows lysates of human placenta tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human MMP-14/MT1-MMP Monoclonal Antibody (Catalog # MAB918) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # <a class=

HAF018). Specific bands were detected for MMP-14/MT1-MMP at approximately 50 kDa and 62 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of human placenta tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human MMP-14/MT1-MMP Monoclonal Antibody (Catalog # MAB918) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for MMP-14/MT1-MMP at approximately 50 kDa and 62 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

CTS003) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox">

MMP-14 was detected in immersion fixed paraffin-embedded sections of human benign nodular hyperplasia using 25 µg/mL Mouse Anti-Human MMP-14 Monoclonal Antibody (Catalog # MAB918) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-AEC Cell & Tissue Staining Kit (red; Catalog # <a class=

CTS003) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB918U-100UG
Alexa Fluor 405	FAB918V-100UG
Alexa Fluor 488	FAB918G-100UG
Alexa Fluor 532	FAB918X-100UG
Alexa Fluor 594	FAB918T-100UG
Alexa Fluor 647	FAB918R-100UG
Alexa Fluor 700	FAB918N-100UG
Alexa Fluor 750	FAB918S-100UG
Alexa Fluor Plus 405	FAB918AFP405-100UG
Alexa Fluor Plus 488	FAB918AFP488-100UG
Alexa Fluor Plus 555	FAB918AFP555-100UG
Alexa Fluor Plus 594	FAB918AFP594-100UG
Alexa Fluor Plus 647	FAB918AFP647-100UG
Alexa Fluor Plus 680	FAB918AFP680-100UG

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB918-SP

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AF2967

MMP-7 Antibody [Unconjugated]

(11 Publications)

100 μg

AF2967

CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of cells in intestinal glands. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_lightbox">

MMP‑7 was detected in perfusion fixed frozen sections of mouse small intestine using Goat Anti-Mouse MMP‑7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2967) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # <a class=

CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of cells in intestinal glands. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." alt="MMP‑7 was detected in perfusion fixed frozen sections of mouse small intestine using Goat Anti-Mouse MMP‑7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2967) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of cells in intestinal glands. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_thumb" />

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

Applications:

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF2967U-100UG
Alexa Fluor 405	AF2967V-100UG
Alexa Fluor 488	AF2967G-100UG
Alexa Fluor 532	AF2967X-100UG
Alexa Fluor 594	AF2967T-100UG
Alexa Fluor 647	AF2967R-100UG
Alexa Fluor 700	AF2967N-100UG
Alexa Fluor 750	AF2967S-100UG
Alexa Fluor Plus 405	AF2967AFP405-100UG
Alexa Fluor Plus 488	AF2967AFP488-100UG
Alexa Fluor Plus 555	AF2967AFP555-100UG
Alexa Fluor Plus 594	AF2967AFP594-100UG
Alexa Fluor Plus 647	AF2967AFP647-100UG
Alexa Fluor Plus 680	AF2967AFP680-100UG

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF2967-SP

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Category

Protein/Target

Applications

Reactivity

Citation

Antibody Validation

Reviews

Host

Available Conjugates

Format

Marker

Modification

Clonality

Clone

Gene Symbol

Images Type

Isotype

Preservative

Research Areas

Has Sample Size

Promotional Item

Species Glossary

MMP-1 Antibody (36665) [Unconjugated]

MMP-14/MT1-MMP Antibody (3-F7)

MMP-7 Antibody (111433) [Unconjugated]

MMP-3 Antibody [Unconjugated]

MMP-1 Antibody [Unconjugated]

MMP-3 Antibody - BSA Free

MMP-14/MT1-MMP Antibody (128527) [Unconjugated]

MMP-7 Antibody [Unconjugated]

MMP-1 Antibody (6A5) - BSA Free

MMP-3 Antibody (50647) [Unconjugated]

MMP-14/MT1-MMP Antibody (5H2) [Unconjugated]

MMP-7 Antibody [Unconjugated]