Recombinant Human MIA2 Protein

Simple Western: Human MIA2 Protein [NBP2-34986]

• Reactivity: Hu
• Applications: PAGE
• Format: Carrier-Free
• Concentration: LYOPH

Recombinant Human MIA2 Protein Summary

• Description: A single non-glycosylated polypeptide chain containing 100 amino acids corresponding to MIA2 Source: E. coli

  Uniprot ID: Q96PC5

  Amino Acid Sequence: LESTKLLADL KKCGDLECEA LINRVSAMRD YRGPDCRYLN FTKGEEISVY VKLAGEREDL WAGSKGKEFG YFPRDAVQIE EVFISEEIQM STKESDFLCL

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: MIA2
• Purity: > 97 % pure by SDS-PAGE and HPLC
• Endotoxin Note: Less than 1 EU/ug of MIA2 as determined by LAL method.

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 11.4 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered concentrated solution in 20 mM PB, pH 7.4, 150 mM NaCl.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: > 97 % pure by SDS-PAGE and HPLC
• Reconstitution Instructions: Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/mL.

Alternate Names for Recombinant Human MIA2 Protein

• FLJ22404
• melanoma inhibitory activity 2
• melanoma inhibitory activity protein 2

Background

Melanoma Inhibitor Activity Protein 2 (MIA2) encoded by MIA2 gene in humans, is a secreted cytokine that is highly expressed in liver and weakly in testis. The patients with severe fibrosis or inflammation and chronic hepatitis have higher level of MIA2 than other. Levels of MIA2 may severe as a clinically utility marker for diagnosis of hepatic disease activity and severity. The MIA2 is a member of the MIA/OTOR family, which also includes MIA, OTOR, and TANGO, and they share a Src homology-3 (SH3)-like domain.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: MIA2
• Uniprot:
  • Q96PC5(Human)