Lipase I Antibody [Alexa Fluor® 488]

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alexa Fluor 488

Lipase I Antibody [Alexa Fluor® 488] Summary

• Immunogen: E. coli-derived recombinant human Lipase I
  Arg14-His154
  Accession # Q6XZB0
• Specificity: Detects human Lipase I in Western blots.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Lipase I Antibody [Alexa Fluor® 488]

• Cancer/testis antigen 17
• CT17
• CT17membrane-associated phospholipase A1 beta
• EC 3.1.1
• EC 3.1.1.-
• EC 3.1.1.3
• EC 3.1.1.34
• Lipase I
• lipase, member I
• LIPI
• LPD lipase
• LPDL
• LPDLlipase member I
• Membrane-associated phosphatidic acid-selective phospholipase A1-beta
• mPA-PLA1 beta
• PRED5

Background

Lipase I (Lipase member I; also mPA‑PLA₁ beta  LPDL and PLA1C) is a 55 kDa member of the PLA₁ subfamily, pancreatic lipase family, AB hydrolase superfamily of enzymes. It is expressed by sperm, where it is apparently secreted and subsequently binds to membrane-associated HSPGs. Lipase I hydrolyzes phosphatidic acid to generate 2‑acyl‑LPA, which then activates one of four G-protein coupled receptors. Human Lipase I precursor is 460 amino acids (aa) in length. It contains a 15 aa signal sequence, followed by an active catalytic site composed of Ser159, Asp183 and His268. Lipase I is known to be phosphorylated on Ser25. There are two isoform variants, both of which show a 34 aa substitution for aa 1‑13, and only one of which shows an eight aa substitution for aa 245‑460. Over aa 14‑154, human Lipase I shares 61% aa identity with mouse Lipase I.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.