Recombinant Human Lgi1 GST (N-Term) Protein

Western Blot: Recombinant Human Lgi1 Protein [H00009211-P01] - The human recombinant LGI1 protein is recognized by a commercial antibody against LGI1. Lane 2: 0.4 ug; Lane 3: 0.2 ug). Lane 4: no protein. Image from verified customer review.

Recombinant Human Lgi1 Protein [H00009211-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.

• Reactivity: Hu
• Applications: WB, ELISA, PA, AP

Recombinant Human Lgi1 GST (N-Term) Protein Summary

• Description: A recombinant protein with GST tag at N-terminal corresponding to the amino acids 1-557 of Human LGI1 full-length ORF
  
  

  Source: Wheat Germ (in vitro)

  Amino Acid Sequence: MESERSKRMGNACIPLKRIAYFLCLLSALLLTEGKKPAKPKCPAVCTCTKDNALCENARSIPRTVPPDVISLSFVRSGFTEISEGSFLFTPSLQLLLFTSNSFDVISDDAFIGLPHLEYLFIENNNIKSISRHTFRGLKSLIHLSLANNNLQTLPKDIFKGLDSLTNVDLRGNSFNCDCKLKWLVEWLGHTNATVEDIYCEGPPEYKKRKINSLSSKDFDCIITEFAKSQDLPYQSLSIDTFSYLNDEYVVIAQPFTGKCIFLEWDHVEKTFRNYDNITGTSTVVCKPIVIETQLYVIVAQLFGGSHIYKRDSFANKFIKIQDIEILKIRKPNDIETFKIENNWYFVVADSSKAGFTTIYKWNGNGFYSHQSLHAWYRDTDVEYLEIVRTPQTLRTPHLILSSSSQRPVIYQWNKATQLFTNQTDIPNMEDVYAVKHFSVKGDVYICLTRFIGDSKVMKWGGSSFQDIQRMPSRGSMVFQPLQINNYQYAILGSDYSFTQVYNWDAEKAKFVKFQELNVQAPRSFTHVSINKRNFLFASSFKGNTQIYKHVIVDLSA

• Preparation Method: in vitro wheat germ expression system
• Details of Functionality: This protein was produced in an in vitro wheat germ expression system that should preserve correct conformational folding that is necessary for biological function. While it is possible that this protein could display some level of activity, the functionality of this protein has not been explicitly measured or validated.
• Source: Wheat germ
• Protein/Peptide Type: Full Length Recombinant Protein
• Gene: LGI1
• Purity: >80% by SDS-PAGE and Coomassie blue staining

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunoaffinity Purification
  • Protein Array
  • Western Blot
• Theoretical MW: 86.68 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: 50 mM Tris-HCl, 10 mM reduced Glutathione, pH 8.0 in the elution buffer.
• Preservative: No Preservative
• Purity: >80% by SDS-PAGE and Coomassie blue staining

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Recombinant Human Lgi1 GST (N-Term) Protein

• ADLTE
• ADPAEF
• ADPEAF
• BB130740
• epilepsy, partial
• Epitempin
• Epitempin-1
• EPT
• EPTleucine-rich glioma-inactivated protein 1
• ETL1
• ETL1epitempin-1
• IB1099
• leucine-rich, glioma inactivated 1
• LGI1

Background

LGI1( AAH22500, 1 a.a. - 558 a.a.) recombinant protein with GST.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

Publications for Lgi1 Recombinant Protein (H00009211-P01)(1)

Publication using H00009211-P01

• Petit-Pedrol M, Sell J, Planaguma J et al. LGI1 antibodies alter Kv1.1 and AMPA receptors changing synaptic excitability, plasticity and memory. Brain. 2018-11-01 [PMID: 30346486]

Review for Lgi1 Recombinant Protein (H00009211-P01) (1)

Average Rating: 4

(Based on 1 review)

reviewed by: Verified Customer SDS-PAGE Control 10/23/2018

Summary

• Application: SDS-PAGE Control
• Lot: I5111

Comments

• Comments: The human recombinant LGI1 protein loaded in a western blot is recognized by a commercial antibody against LGI1.

Bioinformatics

• Gene Symbol: LGI1