KLF17 Antibody

Western blot shows lysates of human testis tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human KLF17 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6845) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for KLF17 at approximately 37 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

KLF17 Antibody Summary

• Immunogen: E. coli-derived recombinant human KLF17
  Met1-Gln228
  Accession # Q5JT82
• Specificity: Detects human KLF17 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) KLF1, rhKLF2, rhKLF4, rhKLF5, rhKLF6, rhKLF10, and rhKLF12 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: KLF17
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for KLF17 Antibody

• FLJ40160
• KLF17
• Kruppel-like factor 17
• Zfp393
• Zinc finger protein 393Krueppel-like factor 17
• ZNF393
• ZNF393novel zinc-finger protein

Background

KLF17 (Kruppel-like factor 17) is a 38-39 kDa member of the Sp1 C2H2-type zinc-finger protein family. Based on mouse studies, it is expressed in germ cells and likely plays a role in spermatid and oocyte differentiation. It is also found to be downregulated in tumors where, if present, it normally suppresses Id1 (Inhibitor of DNA binding 1), a transcription factor associated with tumorigenesis. Human KLF17 is 389 amino acids (aa) in length and contains three zinc-finger motifs (aa 283‑365). There is one potential alternate splice form that shows a 19 aa substitution for aa 97-389. Over aa 1-228, human KLF17 shares only 33% aa identity with mouse ZFP393/KLF17.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: KLF17
• Uniprot:
  • Q5JT82(Human)