Intrinsic Factor ELISA Kit (Colorimetric)

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Product Details

Summary
Applications ELISA, NULL
Suitable Sample Type
Plasma, Serum
Standard Curve Range
6.3 to 100 U/mL
Sensitivity
0.5 U/mL

Order Details

Intrinsic Factor ELISA Kit (Colorimetric) Summary

Description
Intrinsic Factor Ab ELISA Kit is an indirect solid phase ELISA for the quantitative measurement of IgG class autoantibodies against intrinsic factor in human serum or plasma.
Standard Curve Range
6.3 to 100 U/mL
Sensitivity
0.5 U/mL
Assay Type
Indirect ELISA
Inter-Assay
CV% < 3.8-5.6%
Intra-Assay
CV% < 3.2-4.5%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
CBLIF

Applications/Dilutions

Dilutions
  • ELISA
  • Quantification

Packaging, Storage & Formulations

Storage
Store at 4C.

Kit Components

Components
  1. Calibrator A-F (0, 6.3, 12.5, 25, 50, 100 U/mL): Containing serum/buffer matrix (PBS, BSA,detergent, NaN3 0.09%), yellow. Ready to use.
  2. Control positive (1) and negative (2), containing intrinsic factor antibodies in a serum/buffermatrix (PBS, BSA, detergent, NaN3 0.09%), yellow, Ready to use. The concentration isspecified on the certificate of analysis.
  3. Divisible microplate: Consisting of 12 modules of 8 wells each. Ready to use.
  4. Enzyme conjugate; containing anti-human IgG antibodies, HRP labelled; PBS, BSA,detergent, preservative ProClin 300 0.05%, light red. Ready to use.
  5. Sample Buffer P, containing PBS, BSA, detergent, preservative NaN3 0.09%, yellow, 5x conc.
  6. Stop solution; contains acid. Ready to use.
  7. TMB substrate; containing 3,3’,5,5’-Tetramethylbenzidin, colorless. Ready to use.
  8. Wash Solution, containing Tris, detergent, preservative NaN3 0.09%; 50 x conc.

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Intrinsic Factor ELISA Kit (Colorimetric)

  • CBLIF
  • Cobalamin binding intrinsic factor
  • Gastric intrinsic factor
  • GIF
  • IF
  • IFMH
  • INF
  • Intrinsic factor
  • Precursor
  • TCN3

Background

Intrinsic factor is a glycoprotein secreted by gastric mucosal cells that is essential for vitamin B-12 absorption.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol CBLIF