Intrinsic Factor ELISA Kit (Colorimetric)

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Product Details

Summary
Applications ELISA
Suitable Sample Type
Plasma, Serum
Standard Curve Range
6.3 to 100 U/mL
Sensitivity
1 U/mL

Order Details

Intrinsic Factor ELISA Kit (Colorimetric) Summary

Standard Curve Range
6.3 to 100 U/mL
Sensitivity
1 U/mL
Assay Type
Sandwich ELISA
Inter-Assay
CV% < 3.8-5.6%
Intra-Assay
CV% < 3.2-4.5%
Sample Volume
10 uL
Kit Type
ELISA Kit (Colorimetric)

Applications/Dilutions

Application Notes
Intrinsic Factor Ab ELISA Kit is an indirect solid phase ELISA for the quantitative measurement of IgG class autoantibodies against intrinsic factor in human serum or plasma.

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Sample Buffer P, containing PBS, BSA, detergent, preservative NaN3 0.09%, yellow, 5x conc.
  2. TMB substrate; containing 3,3’,5,5’-Tetramethylbenzidin, colorless. Ready to use.
  3. Stop solution; contains acid. Ready to use.
  4. Wash Solution, containing Tris, detergent, preservative NaN3 0.09%; 50 x conc.
  5. Control positive (1) and negative (2), containing intrinsic factor antibodies in a serum/buffermatrix (PBS, BSA, detergent, NaN3 0.09%), yellow, Ready to use. The concentration isspecified on the certificate of analysis.
  6. Calibrator A-F (0, 6.3, 12.5, 25, 50, 100 U/mL): Containing serum/buffer matrix (PBS, BSA,detergent, NaN3 0.09%), yellow. Ready to use.
  7. Divisible microplate: Consisting of 12 modules of 8 wells each. Ready to use.
  8. Enzyme conjugate; containing anti-human IgG antibodies, HRP labelled; PBS, BSA,detergent, preservative ProClin 300 0.05%, light red. Ready to use.

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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