Human IFN-omega/IFNW1 ELISA Kit (Colorimetric)

Human IFN-omega/IFNW1 ELISA Kit [NBP1-91178] - Data for Human IFN-omega Platinum ELISA

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Cell culture supernatant, serum, plasma (EDTA, citrate, heparin), buffered solution
• Standard Curve Range: 4.7 - 300 pg/mL
• Sensitivity: 1.5 pg/mL

Human IFN-omega/IFNW1 ELISA Kit (Colorimetric) Summary

• Description: The human IFN-omega ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human IFN-omega.
• Specificity: The interference of circulating factors of the immune systeme was evaluated by spiking these proteins at physiologically relevant concentrations into human serum and cell culture supernatant. There was no crossreactivity detected, notably not with IFN alpha and IFN gamma.
• Standard Curve Range: 4.7 - 300 pg/mL
• Sensitivity: 1.5 pg/mL
• Assay Type: Sandwich ELISA
• Inter-Assay: CV% < 6.80%
• Intra-Assay: CV% < 4.90%
• Spike Recovery: The recovery ranged from 76% to 120% with an overall mean recovery of 91%. Please see protocol for more specific Spike Recovery details.
• Sample Volume: 25 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: IFNW1

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human IFN-omega/IFNW1 ELISA Kit (Colorimetric)

• IFN-omega 1, interferon omega-1
• IFNomega
• IFN-omega
• IFNW1
• Interferon alpha-II-1
• interferon omega-1
• interferon, omega 1

Background

In the human genome, the IFN-omega gene family consists of seven members located on chromosome 9. However, only one of these genes is functional giving rise to the IFN-omega protein, whereas the others are non-functional pseudogenes. The IFN-omega gene is not expressed in unstimulated cells. Viral infection results in expression of the gene giving rise to the N-glycosylated protein consisting of 172 or 174 amino acids and an apparent molecular mass of about 25kDa. A single carbohydrate group consists mainly of biantennary complex oligosaccharides with variable amounts of N-acetyl neuraminic acid. In quantitative terms, IFN-omega is a major component of human leukocyte IFN; with a contribution to its total antiviral activity estimated to be in the range of 10 -15 %.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for IFN-omega/IFNW1 ELISA Kit (NBP1-91178). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: IFNW1