Human IFN-omega/IFNW1 ELISA Kit (Colorimetric) Summary
| Description |
The human IFN-omega ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human IFN-omega. |
| Specificity |
The interference of circulating factors of the immune systeme was evaluated by spiking these proteins at physiologically relevant concentrations into human serum and cell culture supernatant. There was no crossreactivity detected, notably not with IFN alpha and IFN gamma. |
| Standard Curve Range |
4.7 - 300 pg/mL |
| Sensitivity |
1.5 pg/mL |
| Assay Type |
Sandwich ELISA |
| Inter-Assay |
CV% < 6.80% |
| Intra-Assay |
CV% < 4.90% |
| Spike Recovery |
The recovery ranged from 76% to 120% with an overall mean recovery of 91%. Please see protocol for more specific Spike Recovery details. |
| Sample Volume |
25 uL |
| Kit Type |
ELISA Kit (Colorimetric) |
| Gene |
IFNW1 |
Applications/Dilutions
Packaging, Storage & Formulations
| Storage |
Storage of components varies. See protocol for specific instructions. |
Kit Components
|
Components
|
- 1 vial (1.1 ml) monoclonal Coating Antibody to human IFN-omega (100 ug/ml)
- 1 vial (11 ul) Streptavidin-HRP
- 1 vial (30 ul) human IFN-omega Standard protein, 60 ng/ml
- 1 vial (55 ul) Biotin-Conjugate anti-human IFN-omega monoclonal antibody
- 2 vials (50 ml) Sample Diluent
|
Alternate Names for Human IFN-omega/IFNW1 ELISA Kit (Colorimetric)
Background
In the human genome, the IFN-omega gene family consists of seven members located on chromosome 9. However, only one of these genes is functional giving rise to the IFN-omega protein, whereas the others are non-functional pseudogenes. The IFN-omega gene is not expressed in unstimulated cells. Viral infection results in expression of the gene giving rise to the N-glycosylated protein consisting of 172 or 174 amino acids and an apparent molecular mass of about 25kDa. A single carbohydrate group consists mainly of biantennary complex oligosaccharides with variable amounts of N-acetyl neuraminic acid. In quantitative terms, IFN-omega is a major component of human leukocyte IFN; with a contribution to its total antiviral activity estimated to be in the range of 10 -15 %.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
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FAQs for IFN-omega/IFNW1 ELISA Kit (NBP1-91178). (Showing 1 - 1 of 1 FAQ).
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wondering what the difference is between your quantikine and duo set elisas?
- Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.
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