Hsp70 interacting protein HIP Antibody

Western Blot: Hsp70 interacting protein HIP Antibody [NB120-13490] - analysis of rat testis tissue extract, probed with Hsp70 interacting protein HIP [NB120-13490] polyclonal antibody.

Product Discontinued

Hsp70 interacting protein HIP Antibody Summary

• Immunogen: Recombinant Histidine 6 fusion protein (Rat).
• Localization: Cytoplasmic
• Specificity: This detects both recombinant rat HSC70 Interacting Protein HIP as well as the native protein in tissue extracts.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: ST13
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1:1000
• Application Notes: This antibody is useful in Immunohistochemistry and Western Blot. For WB use at a dilution of 1:1000 (human and bovine) or 1:5000 (mouse and rat). 1:5000 dilution was sufficient to detect 50ng of recombinant rat protein. Detects a band of approximately 58 kDa (predicted molecular weight: 42 kDa).

Reactivity Notes

Cross-reacts with Human, Mouse, Rat and Cow. Whilst reactivity with human and bovine protein is detected, this reactivity is much weaker than that observed with samples from rat or mouse origin.Not yet tested in other species.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 50% Glycerol
• Preservative: 0.09% Sodium Azide
• Purity: Protein A purified

Alternate Names for Hsp70 interacting protein HIP Antibody

• AAG2
• aging-associated protein 2
• FAM10A1FAM10A4
• heat shock 70kD protein binding protein
• Hip
• HIPFLJ27260
• HOP
• hsc70-interacting protein
• Hsp70-interacting protein
• HSPABP1
• P48MGC129952
• PRO0786
• Progesterone receptor-associated p48 protein
• Protein FAM10A1
• Putative tumor suppressor ST13
• Renal carcinoma antigen NY-REN-33
• SNC6HSPABP
• suppression of tumorigenicity 13 (colon carcinoma) (Hsp70 interacting protein)
• suppression of tumorigenicity 13 (colon carcinoma) (Hsp70-interacting protein)
• Suppression of tumorigenicity 13 protein

Background

Hip (Hsc70-interacting protein) is a cytosolic protein that exists as a homo-oligomer and participates in the regulation of Hsc70 in eukaryotic cells. Hip interacts with the ATPase domain of Hsc70 molecules through its tetratricopeptide repeats and flanking charged alpha-helices. Upon binding to Hsc70, Hip stabilizes the ADP-bound state of Hsc70, a conformation that has a high affinity for unfolded substrate proteins. Together with Hsp40 and BAG-1, Hip may regulate the chaperone activity of Hsc70 by stabilizing the chaperone-substrate complex.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ST13
• Entrez:
  • 6767(Human)
  • 70356(Mouse)
  • 81800(Rat)