Hsp70 interacting protein HIP Antibody (2G6)

Immunocytochemistry/Immunofluorescence: Hsp70 interacting protein HIP Antibody (2G6) [NB120-2813] - Analysis of HIP in HeLa cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a HIP monoclonal antibody at a dilution of 1:20 overnight at 4C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. HIP staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.

Immunocytochemistry/Immunofluorescence: Hsp70 interacting protein HIP Antibody (2G6) [NB120-2813] - A431 cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a HIP monoclonal antibody at a dilution of 1:100 overnight at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. HIP staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.

Immunocytochemistry/Immunofluorescence: Hsp70 interacting protein HIP Antibody (2G6) [NB120-2813] - Analysis of HIP in NIH-3T3 cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a HIP monoclonal antibody at a dilution of 1:200 overnight at 4C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. HIP staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.

Immunocytochemistry/Immunofluorescence: Hsp70 interacting protein HIP Antibody (2G6) [NB120-2813] - Analysis of HIP in A431 cells. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with a HIP monoclonal antibody at a dilution of 1:100 overnight at 4C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. HIP staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Images were taken at 60X magnification.

Product Discontinued

Hsp70 interacting protein HIP Antibody (2G6) Summary

• Immunogen: Recombinant human Hip protein.
• Localization: Cytoplasmic
• Specificity: NB120-2813 detects heat shock cognate protein 70 kDa (HSC70)-interacting protein (Hip) from human, chicken and rabbit samples.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Mouse
• Gene: ST13
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1 - 2 ug/ml
  • Immunoprecipitation 1:10 - 1:500
  • Western Blot 1 ug/ml
• Application Notes: WB: Detects an approx. 48 kDa protein representing Hip from rabbit reticulocyte lysate.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Ascites diluted with PBS
• Preservative: 0.05% Sodium Azide
• Purity: Unpurified

Alternate Names for Hsp70 interacting protein HIP Antibody (2G6)

• AAG2
• aging-associated protein 2
• FAM10A1FAM10A4
• heat shock 70kD protein binding protein
• Hip
• HIPFLJ27260
• HOP
• hsc70-interacting protein
• Hsp70-interacting protein
• HSPABP1
• P48MGC129952
• PRO0786
• Progesterone receptor-associated p48 protein
• Protein FAM10A1
• Putative tumor suppressor ST13
• Renal carcinoma antigen NY-REN-33
• SNC6HSPABP
• suppression of tumorigenicity 13 (colon carcinoma) (Hsp70 interacting protein)
• suppression of tumorigenicity 13 (colon carcinoma) (Hsp70-interacting protein)
• Suppression of tumorigenicity 13 protein

Background

Steroid receptors are ligand-dependent intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate hormone. Prior to activation, steroid receptors associate with a number of different proteins in both a stable and transient fashion. Steroid receptor complex proteins include heat shock proteins (HSP90, HSP70), immunosuppressant binding proteins called immunophilins (the FK506 binding proteins, FKBP52 & FKBP54 and the cyclosporin binding protein, CyP-40) and three other proteins termed p23, p60 and heat shock cognate protein 70 kDa (HSC70)-interacting protein (Hip), also known as p48. Hip, along with HSP70 and p60, combine with steroid receptors as transient intermediate complex members. In vitro studies have suggested that Hip transiently interacts with progesterone receptor in a cell free system prior to hormone binding competency and dissociates from the receptor complex with the binding of an HSP90:Immunophilin:p23 subcomplex which then results in a hormone-binding-competent receptor complex. While neither its exact function nor mechanism of action have been identified, Hip appears to be an important factor in steroid receptor function. Hip also appears to regulate the function of HSC70 in an ATP hydrolysis and HSP40 dependent fashion.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Hsp70 interacting protein HIP Antibody (NB120-2813)(1)

Publication using NB120-2813

• Barker BL, Benovic J. G protein-coupled receptor kinase 5 phosphorylation of hip regulates internalization of the chemokine receptor CXCR4 Biochemistry 2011-08-16 [PMID: 21728385] (IP, WB, Human)

Bioinformatics

• Gene Symbol: ST13
• Entrez:
  • 6767(Human)
• Uniprot:
  • P50502(Human)