HPCL Antibody

Western Blot: HPCL Antibody [NBP1-31571] - Sample(30 ug whole cell lysate)A:293T 7.5% SDS PAGE, antibody diluted at 1:1000.

Immunocytochemistry/Immunofluorescence: HPCL Antibody [NBP1-31571] - Paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.

Immunohistochemistry-Paraffin: HPCL Antibody [NBP1-31571] - HPCL antibody detects HPCL protein at cytosol on human hepatoma by immunohistochemical analysis. Sample: Paraffin-embedded hepatoma.HPCL antibody dilution: 1:500. Antigen Retrieval: Trilogy (EDTA based, pH 8.0) buffer, 15min

Western Blot: HPCL Antibody [NBP1-31571] - Sample (50 ug of whole cell lysate) A: mouse liver 7. 5% SDS PAGE; antibody diluted at 1:1000.

Immunohistochemistry-Paraffin: HPCL Antibody [NBP1-31571] - HPCL antibody detects HPCL protein at cytosol on human normal liver by immunohistochemical analysis. Sample: Paraffin-embedded human normal liver.HPCL antibody dilution: 1:500. Antigen Retrieval: Trilogy (EDTA based, pH 8.0) buffer, 15min

Product Discontinued

HPCL Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the C-terminus region of human HACL1. The exact sequence is proprietary.
• Localization: Peroxisome
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: HACL1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol
• Preservative: 0.01% Thimerosal
• Purity: Immunogen affinity purified

Alternate Names for HPCL Antibody

• 1600020H07Rik
• 2,2-HPCL
• 2-hydroxyacyl-CoA lyase 1
• 2-hydroxyphytanoyl-CoA lyase2-hydroxyphytanol-CoA lyase
• EC 4.1
• HPCL
• PHYH2FLJ55041
• Phytanoyl-CoA 2-hydroxylase 2
• phytanoyl-CoA hydroxylase 2

Background

HPCL catalyzes a carbon-carbon cleavage reaction; cleaves a 2-hydroxy-3-methylacyl-CoA into formyl-CoA and a 2-methyl-branched fatty aldehyde

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: HACL1