Histone H3.1 Antibody

Images

 
Western Blot: Histone H3.1 Antibody [NBP2-75524] - Analysis of Histone H3.1 on F9 cell lysate using anti-Histone H3.1 antibody at 1/1,000 dilution.
Immunocytochemistry/ Immunofluorescence: Histone H3.1 Antibody [NBP2-75524] - Staining Histone H3.1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised ...read more
Immunohistochemistry-Paraffin: Histone H3.1 Antibody [NBP2-75524] - Analysis of paraffin-embedded human liver tissue using anti-Histone H3.1 antibody. Counter stained with hematoxylin.
Immunocytochemistry/ Immunofluorescence: Histone H3.1 Antibody [NBP2-75524] - Staining Histone H3.1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised ...read more
Immunocytochemistry/ Immunofluorescence: Histone H3.1 Antibody [NBP2-75524] - Staining Histone H3.1 in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

Histone H3.1 Antibody Summary

Immunogen
Synthetic peptide of N-terminal human Histone H3.1.
Localization
Nucleus.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HIST1H3E
Purity
Peptide affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin 1:50-1:200
Theoretical MW
15 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
TBS (pH7.4), 0.5%BSA, 50%Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Peptide affinity purified

Alternate Names for Histone H3.1 Antibody

  • H3 histone family, member D
  • H3.1HIST1H3D
  • H3/d
  • H3FA
  • H3FB
  • H3FC
  • H3FDHIST1H3F
  • H3FF
  • H3FH
  • H3FI
  • H3FJ
  • H3FK
  • H3FL
  • HIST1H3A
  • HIST1H3B
  • HIST1H3C
  • HIST1H3G
  • HIST1H3H
  • HIST1H3I
  • HIST1H3J
  • histone 1, H3e
  • histone cluster 1, H3e
  • Histone H3.1
  • Histone H3/a
  • Histone H3/b
  • Histone H3/c
  • Histone H3/d
  • Histone H3/f
  • Histone H3/h
  • Histone H3/i
  • Histone H3/j
  • Histone H3/k
  • Histone H3/l

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Blogs on Histone H3.1.

H3.1 - A core histone essential for genome storage and organization
Histones are the main protein component of chromatin and are essential for the storage and compaction of the genome. DNA wraps around histone oligomers to make up nucleosomes, the individual subunits of chromatin. By altering the accessibility of t...  Read full blog post.

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Bioinformatics

Gene Symbol HIST1H3E