![Western Blot: Histone H2AY/macroH2A.1 Antibody [NBP2-76947] - Western blot analysis of macroH2A.1 on different lysates using anti-macroH2A.1 antibody at 1/500 dilution.Positive control:Lane1: mouse pancreas Lane2: SiHaLane3: PC-3M](http://images.novusbio.com/fullsize2/Histone-H2AY-macroH2A.1-Antibody-Western-Blot-NBP2-76947-img0001.jpg "Western Blot: Histone H2AY/macroH2A.1 Antibody [NBP2-76947] - Western blot analysis of macroH2A.1 on different lysates using anti-macroH2A.1 antibody at 1/500 dilution.Positive control:Lane1: mouse pancreas Lane2: SiHaLane3: PC-3M")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, Flow, IHC, IHC-P |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Immunogen | Recombinant protein within human macroH2A.1 aa 200-400. |
| Localization | Nucleus. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | H2AFY |
| Purity | Peptide affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Theoretical MW | 40 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | TBS (pH7.4), 0.5%BSA, 50% Glycerol |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Peptide affinity purified |
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | H2AFY |
![Immunohistochemistry: Histone H2AY/macroH2A.1 Antibody [NBP2-76947] - Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-macroH2A.1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize2/Histone-H2AY-macroH2A.1-Antibody-Immunohistochemistry-NBP2-76947-img0004.jpg "Immunohistochemistry: Histone H2AY/macroH2A.1 Antibody [NBP2-76947] - Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-macroH2A.1 antibody. Counter stained with hematoxylin.")
![Flow Cytometry: Histone H2AY/macroH2A.1 Antibody [NBP2-76947] - Flow cytometric analysis of SiHa cells with macroH2A.1 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.](http://images.novusbio.com/fullsize2/Histone-H2AY-macroH2A.1-Antibody-Flow-Cytometry-NBP2-76947-img0005.jpg "Flow Cytometry: Histone H2AY/macroH2A.1 Antibody [NBP2-76947] - Flow cytometric analysis of SiHa cells with macroH2A.1 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.")
![Immunohistochemistry: Histone H2AY/macroH2A.1 Antibody [NBP2-76947] - Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-macroH2A.1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize2/Histone-H2AY-macroH2A.1-Antibody-Immunohistochemistry-NBP2-76947-img0002.jpg "Immunohistochemistry: Histone H2AY/macroH2A.1 Antibody [NBP2-76947] - Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-macroH2A.1 antibody. Counter stained with hematoxylin.")
![Immunohistochemistry: Histone H2AY/macroH2A.1 Antibody [NBP2-76947] - Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-macroH2A.1 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize2/Histone-H2AY-macroH2A.1-Antibody-Immunohistochemistry-NBP2-76947-img0003.jpg "Immunohistochemistry: Histone H2AY/macroH2A.1 Antibody [NBP2-76947] - Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-macroH2A.1 antibody. Counter stained with hematoxylin.")
![Western Blot: Histone H2a Antibody [NB100-56346] - Histone H2A in MCF-7 and HCT-116 cells. Image from verified customer review.](http://images.novusbio.com/images2/Histone-H2a-Antibody-Western-Blot-NB100-56346-img0004.jpg)
![Immunohistochemistry-Paraffin: Histone H2a Antibody [NB100-56346] - Human breast probed with Histone H2A antibody at 5 ug/ml. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.](http://images.novusbio.com/images2/Histone-H2a-Antibody-Immunohistochemistry-Paraffin-NB100-56346-img0006.jpg)
![Western Blot: Histone H2a Antibody [NB100-56346] - Analysis of Histone H2A in human PBMC lysate probed with Histone H2A antibody.](http://images.novusbio.com/images2/Histone H2a Antibody-Western Blot-NB100-56346-img0002.jpg)
![Western Blot: PARP Antibody (194C1439) - Cleaved [NB100-56599] - Analysis of cleaved PARP in staurosporine-treated Jurkat cells at various time points, antibody at 2 ug/mL. The band corresponding to cleaved PARP is only seen in the treated samples. Anti-mouse Ig HRP conjugate was used in this test.](http://images.novusbio.com/images2/PARP-Antibody-(194C1439)---Cleaved-Western-Blot-NB100-56599-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: PARP Antibody (194C1439) - Cleaved [NB100-56599] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-PARP Antibody (194C1439) at 5 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/images2/PARP-Antibody-(194C1439)---Cleaved-Immunocytochemistry-Immunofluorescence-NB100-56599-img0005.jpg)
![Western Blot: PARP Antibody (194C1439) - Cleaved [NB100-56599] - Analysis using 293T lysates expressing PARP. Image from verified customer review.](http://images.novusbio.com/images2/PARP-Antibody-(194C1439)---Cleaved-Western-Blot-NB100-56599-img0003.jpg)
![Western Blot: Histone H3 [Trimethyl Lys9] Antibody (6F12-H4) [NBP1-30141] - Whole cell protein from human HeLa, A431, mouse Neuro2A and rat PC12 cells was separated on a 4-20% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 1.0 ug/ml anti-Histone h3 in block buffer and detected with an anti-mouse HRP secondary antibody using chemiluminescence. Observed molecular weight is ~15 kDa.](http://images.novusbio.com/images2/Histone-H3-[Trimethyl-Lys9]-Antibody-(6F12-H4)-Western-Blot-NBP1-30141-img0012.jpg)
![Immunohistochemistry-Paraffin: Histone H3 [Trimethyl Lys9] Antibody (6F12-H4) [NBP1-30141] - Histone 3 was detected in immersion fixed paraffin-embedded sections of human colon cancer using anti-human mouse monoclonal antibody (Catalog # NBP1-30141) at 1:1000 dilution overnight at 4C. Tissue was stained using the VisuCyte anti-mouse HRP polymer detection reagent (Catalog # VC001) with DAB chromogen (brown) and counterstained with hematoxylin (blue). Images may not be copied, printed or otherwise disseminated without express written permission of Novus Biologicals a bio-techne brand.](http://images.novusbio.com/images2/Histone-H3-[Trimethyl-Lys9]-Antibody-(6F12-H4)-Immunohistochemistry-Paraffin-NBP1-30141-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H3 [Trimethyl Lys9] Antibody (6F12-H4) [NBP1-30141] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton X-100. The cells were incubated with anti-Histone H3 [Trymethyl Lys9] (6F12-H4) at 2 ug/ml overnight at 4C and detected with an anti-mouse DyLight 488 (green) at a 1:500 dilution. Nuclei were counterstained with DAPI (blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/images2/Histone-H3-[Trimethyl-Lys9]-Antibody-(6F12-H4)-Immunocytochemistry-Immunofluorescence-NBP1-30141-img0011.jpg)
![Western Blot: HIST1H4F Overexpression Lysate (Adult Normal) [NBP2-07996] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for HIST1H4F.](http://images.novusbio.com/images/HIST1H4F Overexpression Lysate (Adult Normal)-Western Blot-NBP2-07996-img0001.jpg)
![Western Blot: H4/n Antibody (3E7H2) [NBP2-52486] - Analysis using HIST2H4A(20Me) mouse mAb against THP-1 (1) cell lysate.](http://images.novusbio.com/images2/H4-n-Antibody-(3E7H2)-Western-Blot-NBP2-52486-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: H4/n Antibody (3E7H2) [NBP2-52486] - Analysis of HeLa cells using HIST2H4A(20Me) mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.](http://images.novusbio.com/images2/H4-n-Antibody-(3E7H2)-Immunohistochemistry-NBP2-52486-img0003.jpg)
![Immunohistochemistry-Paraffin: H4/n Antibody (3E7H2) [NBP2-52486] - Analysis of paraffin-embedded stomach cancer tissues using HIST2H4A(20Me) mouse mAb with DAB staining.](http://images.novusbio.com/images2/H4-n-Antibody-(3E7H2)-Immunohistochemistry-Paraffin-NBP2-52486-img0005.jpg)
![SDS-Page: Recombinant Human HIST1H4I Protein [H00008294-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/images/qc_test-H00008294-P01-1.jpg)
![Western Blot: Histone H4 [ac Lys5] Antibody [NB21-2024] - Analysis of H4K5ac in NIH 3t3 histone prep.](http://images.novusbio.com/images2/Histone-H4-[ac--Lys5]-Antibody-Western-Blot-NB21-2024-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H4 [ac Lys5] Antibody [NB21-2024] - Histone H4 K5ac antibody was tested in HeLa cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).](http://images.novusbio.com/images2/Histone-H4-[ac--Lys5]-Antibody-Immunocytochemistry-Immunofluorescence-NB21-2024-img0007.jpg)
![Chromatin Immunoprecipitation: Histone H4 [ac Lys5] Antibody [NB21-2024] - 2 ug of NB21-2024 was used to IP DNA from fixed Hela cells alongside a no antibody (No Ab) control. DNA was measured by qRT-PCR and normalized to total input (input=1).](http://images.novusbio.com/images2/Histone-H4-[ac--Lys5]-Antibody-Chromatin-Immunoprecipitation-NB21-2024-img0008.jpg)
![Western Blot: BMI-1 Antibody (LLBmi1-1) [NBP1-96140] - BMI-1 expression in human cell lines (MCF-7, K562 and A459). Image from verified customer review.](http://images.novusbio.com/images2/BMI-1-Antibody-(LLBmi1-1)-Western-Blot-NBP1-96140-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: Bmi1 Antibody (LLBmi1-1) [NBP1-96140] - Bmi1 antibody was tested in U2OS cells with FITC (green). Nuclei and alpha-tubulin were counterstained with Dapi (blue) and Dylight 550 (red).](http://images.novusbio.com/images2/Bmi1-Antibody-(LLBmi1-1)-Immunocytochemistry-Immunofluorescence-NBP1-96140-img0003.jpg)
![Flow Cytometry: BMI-1 Antibody (LLBmi1-1) [NBP1-96140] - An intracellular stain was performed on U2OS cells with BMI-1 [LLBmi1-1] Antibody NBP1-96140 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1.0 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (84540, Thermo Fisher).](http://images.novusbio.com/images2/BMI-1-Antibody-(LLBmi1-1)-Flow-Cytometry-NBP1-96140-img0007.jpg)
![Western Blot: Collagen XI alpha 2 Antibody (473) [NBP2-43728] - Various tissue extracts (50 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with COL11A2 antibody [473] diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/images2/Collagen-XI-alpha-2-Antibody-(473)-Western-Blot-NBP2-43728-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Collagen XI alpha 2 Antibody (473) [NBP2-43728] - A431 cells were fixed in ice-cold MeOH for 5 min. Green: COL11A2 protein stained by COL11A2 antibody [473] diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/images2/Collagen-XI-alpha-2-Antibody-(473)-Immunocytochemistry-Immunofluorescence-NBP2-43728-img0003.jpg)
![Western Blot: Collagen XI alpha 2 Antibody (473) [NBP2-43728] - Analysis of A. 30 ug 293T whole cell lysate/extract B. 30 ug whole cell lysate/extract of human COL11A2-transfected 293T cells (N-terminal fragment of COL11A2 isoform 1) 12 % SDS-PAGE COL11A2 antibody [473] dilution: 1:5000](http://images.novusbio.com/images2/Collagen-XI-alpha-2-Antibody-(473)-Western-Blot-NBP2-43728-img0002.jpg)
![Western Blot: Serpin A7/TBG Antibody (5B11E9) [NBP2-37322] - Western blot analysis using SERPINA7 mAb against HEK293 (1) and SERPINA7 (AA: 168-302)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/images2/Serpin-A7-TBG-Antibody-(5B11E9)-Western-Blot-NBP2-37322-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Serpin A7/TBG Antibody (5B11E9) [NBP2-37322] - Immunofluorescence analysis of A431 cells using SERPINA7 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.](http://images.novusbio.com/images2/Serpin-A7-TBG-Antibody-(5B11E9)-Immunocytochemistry-Immunofluorescence-NBP2-37322-img0004.jpg)
![Immunohistochemistry: Serpin A7/TBG Antibody (5B11E9) [NBP2-37322] - Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using SERPINA7 mouse mAb with DAB staining.](http://images.novusbio.com/images2/Serpin-A7-TBG-Antibody-(5B11E9)-Immunohistochemistry-NBP2-37322-img0007.jpg)
![Western Blot: CBX1 Antibody [NBP2-98756] - Anti-CBX1 rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate. Lane B: MCF7 Whole Cell Lysate. Lane C: A431 Whole Cell Lysate. Lane D: 293T Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 21 kDa](http://images.novusbio.com/images2/CBX1-Antibody-Western-Blot-NBP2-98756-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: CBX1 Antibody [NBP2-98756] - Immunofluorescence staining of CBX1 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human CBX1 polyclonal antibody (dilution ratio 1:1000) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG Secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Nucleus.](http://images.novusbio.com/images2/CBX1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-98756-img0001.jpg)
![Immunohistochemistry-Paraffin: CBX1 Antibody [NBP2-98756] - Immunochemical staining CBX1 in mouse testis with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/images2/CBX1-Antibody-Immunohistochemistry-Paraffin-NBP2-98756-img0005.jpg)
![Western Blot: SPOP Antibody [H00008405-B01P] - Analysis of SPOP expression in transfected 293T cell line by SPOP polyclonal antibody. Lane 1: SPOP transfected lysate(41.14 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/images2/SPOP Antibody-Western Blot-H00008405-B01P-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: SPOP Antibody [H00008405-B01P] - Analysis of purified antibody to SPOP on HeLa cell. (antibody concentration 10 ug/ml)](http://images.novusbio.com/images2/SPOP-Antibody-Immunocytochemistry-Immunofluorescence-H00008405-B01P-img0003.jpg)
![Western Blot: H2A Antibody [NBP1-58095] - Jurkat cell lysate, concentration 2.5 ug/ml.](http://images.novusbio.com/images2/H2A Antibody-Western Blot-NBP1-58095-img0002.jpg)
![Western Blot: QKI/Quaking Antibody [NBP2-20029] - Rat tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with QKI antibody diluted at 1:1000.](http://images.novusbio.com/images2/QKI-Quaking-Antibody-Western-Blot-NBP2-20029-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: QKI/Quaking Antibody [NBP2-20029] - QKI antibody detects QKI protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: QKI protein stained by QKI antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.](http://images.novusbio.com/images2/QKI-Quaking-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-20029-img0004.jpg)
![Immunohistochemistry-Frozen: QKI/Quaking Antibody [NBP2-20029] - QKI antibody detects QKI protein expression by immunohistochemical analysis. Sample: Frozen-sectioned adult mouse cerebellum. Green: QKI protein stained by QKI antibody diluted at 1:250. Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody diluted at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/images2/QKI-Quaking-Antibody-Immunohistochemistry-Frozen-NBP2-20029-img0006.jpg)
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - An intracellular stain was performed on Raji cells with Adiponectin antibody NB100-65810 (blue) and a matched isotype control NBP2-24893 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Dylight488-conjugated anti-rabbit secondary antibody. Image using the Azide Free form of this antibody.](http://images.novusbio.com/images2/Rabbit-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0006.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - An intracellular stain was performed on Raji cells with Adiponectin antibody NB100-65810 (blue) and a matched isotype control NBP2-24893 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Dylight488-conjugated anti-rabbit secondary antibody. Image using the Azide Free form of this antibody.")