Histone H2AY/macroH2A.1 Antibody (JU22-83)

Western Blot: Histone H2AY/macroH2A.1 Antibody (JU22-83) [NBP2-75523] - Analysis of macroH2A.1 on different lysates using anti-macroH2A.1 antibody at 1/500 dilution.Positive control:Lane 1: Hela Lane 2: Mouse placenta tissueLane 3: MCF-7

Immunocytochemistry/Immunofluorescence: Histone H2AY/macroH2A.1 Antibody (JU22-83) [NBP2-75523] - Staining macroH2A.1 in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: Histone H2AY/macroH2A.1 Antibody (JU22-83) [NBP2-75523] - Analysis of paraffin-embedded mouse fallopian tube tissue using anti-macroH2A.1 antibody. Counter stained with hematoxylin.

Immunocytochemistry/Immunofluorescence: Histone H2AY/macroH2A.1 Antibody (JU22-83) [NBP2-75523] - Staining macroH2A.1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: Histone H2AY/macroH2A.1 Antibody (JU22-83) [NBP2-75523] - Staining macroH2A.1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: Histone H2AY/macroH2A.1 Antibody (JU22-83) [NBP2-75523] - Analysis of paraffin-embedded human lung cancer tissue using anti-macroH2A.1 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Histone H2AY/macroH2A.1 Antibody (JU22-83) [NBP2-75523] - Analysis of paraffin-embedded human thyroid gland tissue using anti-macroH2A.1 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Histone H2AY/macroH2A.1 Antibody (JU22-83) [NBP2-75523] - Analysis of paraffin-embedded human placenta tissue using anti-macroH2A.1 antibody. Counter stained with hematoxylin.

Product Discontinued

Histone H2AY/macroH2A.1 Antibody (JU22-83) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human Histone H2AY/macroH2A.1 aa 232-281 / 372. (SwissProt: O75367 Human; SwissProt: Q9QZQ8 Mouse)
• Localization: Nucleus.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: MACROH2A1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:2000
• Theoretical MW: 40 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Histone H2AY/macroH2A.1 Antibody (JU22-83)

• core histone macro-H2A.1
• H2A histone family, member Y
• H2A.y
• H2A/y
• H2AF12M
• H2AFJ
• H2AFY
• Histone H2A.y
• Histone H2AY
• Histone MacroH2A1
• histone macroH2A1.1
• histone macroH2A1.2
• MACROH2A1
• MACROH2A1.1
• macroH2A1.2
• Medulloblastoma Antigen MU-MB-50.205
• mH2A1

Background

macroH2A.1 (H2AFY) is 372 amino acids long, weighs approximately 40 kDa, and is part of a group of basic nuclear proteins called histones, which are responsible for nucleosome structure of the chromosomal fiber in eukaryotes. macroH2a.1 has two shorter isoforms meeasuring 371 and 369 amino acids long, both weighing approximately 39 kDa. Current studies are being done on several diseases and disorders including medulloblastoma, lupus erythematosus, Huntington's disease, immunodeficiency, melanoma, and malaria. macroH2A.1 has also been shown to have interactions with HIST1H4A, HIST1H4B, HIST1H4C, HIST1H4D, and HIST1H4E in pathways such as the chromatin regulation/ acetylation, systemic lupus erythematosus, and ATF-2 transcription factor pathways.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MACROH2A1