Histone H1.3 Antibody (SR2420)

Immunocytochemistry/ Immunofluorescence: Histone H1.3 Antibody (SR2420) [NBP3-27720] - Immunocytochemistry analysis of Histone H1.3 (green) in Hela using NBP3-27720, and DAPI(blue)

Immunohistochemistry-Paraffin: Histone H1.3 Antibody (SR2420) [NBP3-27720] - Immunohistochemistry analysis of paraffin-embedded Human lung cancer. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.

Western Blot: Histone H1.3 Antibody (SR2420) [NBP3-27720] - Western blot analysis of Histone H1.3 in C6, 3T3, Hela lysates

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clone: SR2420
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

Histone H1.3 Antibody (SR2420) Summary

• Description: Novus Biologicals Rabbit Histone H1.3 Antibody (SR2420) (NBP3-27720) is a recombinant monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthetic peptide of human Histone H1.3 (Uniprot #: P16402)
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: H1-3
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:50-1:100
  • Western Blot 1:500-1:1000
• Theoretical MW: 22 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA
• Preservative: 0.01% Sodium Azide
• Purity: Affinity purified

Alternate Names for Histone H1.3 Antibody (SR2420)

• H1 histone family, member 3
• H1.3
• H1F3H1D
• histone 1, H1d
• histone cluster 1, H1d
• histone H1.3
• Histone H1c
• MGC138176

Background

Histones are nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber as they play a critical role in transcription regulation, DNA repair, DNA replication, and chromosomal stability. The histone H1.3 protein is encoded by the HIST1H1D gene and is 221 amino acids long at 22 kDA. H1 histones are critical for the condensation of nucleosome chains into higher order structures. HIST1H1D participates in PKA signaling, cell cycle start of DNA replication in early S phase, apoptosis, and histone modification and is known to interact with genes LMNA, EP300, SUMO1, NOP56 and YWHAQ. Researchers have investigated its role in treacher collins syndrome, meningioma, typhoid fever, and crohn's disease.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: H1-3