Histone H1.3 [p Thr17] Antibody (3K5C10)

Western Blot: Histone H1.3 [p Thr17] Antibody (3K5C10) [NBP3-16927] - Western blot analysis of extracts of various cell lines, using Histone H1.3 Rabbit mAb (NBP3-16927) at 1:1000 dilution.Both NIH/3T3 cells and C6 cells were treated by Nocodazole (50 ng/mL) at 37C for 20 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 1s.

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clone: 3K5C10
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

Histone H1.3 [p Thr17] Antibody (3K5C10) Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthetic phosphorylated peptide around T17 of human Histone H1.3 (NP_005311.1). EKTPV
• Modification: p Thr17
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: H1-3
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500 - 1:1000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol, 0.05% BSA
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for Histone H1.3 [p Thr17] Antibody (3K5C10)

• H1 histone family, member 3
• H1.3
• H1F3H1D
• histone 1, H1d
• histone cluster 1, H1d
• histone H1.3
• Histone H1c
• MGC138176

Background

Histones are nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber as they play a critical role in transcription regulation, DNA repair, DNA replication, and chromosomal stability. The histone H1.3 protein is encoded by the HIST1H1D gene and is 221 amino acids long at 22 kDA. H1 histones are critical for the condensation of nucleosome chains into higher order structures. HIST1H1D participates in PKA signaling, cell cycle start of DNA replication in early S phase, apoptosis, and histone modification and is known to interact with genes LMNA, EP300, SUMO1, NOP56 and YWHAQ. Researchers have investigated its role in treacher collins syndrome, meningioma, typhoid fever, and crohn's disease.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: H1-3