Histone H1.2 Antibody

Images

 
Western Blot: Histone H1.2 Antibody [NBP2-16845] - Sample (30 ug of whole cell lysate) A: HeLa B: HeLa nucleus 12% SDS PAGE gel, diluted at 1:10000.
Immunocytochemistry/ Immunofluorescence: Histone H1.2 Antibody [NBP2-16845] - Confocal immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Histone H1. 2 antibody (Green) at 1:500 dilution. Alpha-tubulin ...read more
Immunohistochemistry-Paraffin: Histone H1.2 Antibody [NBP2-16845] - Immunohistochemical analysis of paraffin-embedded Colon ca, using antibody at 1:250 dilution.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.88 mg/ml

Order Details

Histone H1.2 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human Histone H1.2. The exact sequence is proprietary.
Localization
Nucleus
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
HIST1H1C
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:5000-1:20000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
21 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
0.88 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Histone H1.2 Antibody

  • H1 histone family, member 2
  • H1.2
  • H1F2H1C
  • histone 1, H1c
  • histone cluster 1, H1c
  • histone H1.2
  • Histone H1d
  • MGC3992

Background

Histones are basic nuclear proteins responsible for nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H1 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

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Bioinformatics

Gene Symbol HIST1H1C