![Western Blot: HIST1H3A Antibody [NBP2-41677] - HeLa cells (15 ug) were analyzed by WB blot using the H3 Pan antibody.](http://images.novusbio.com/fullsize/HIST1H3A-C-Term-Antibody-Western-Blot-NBP2-41677-img0001.jpg "Western Blot: HIST1H3A Antibody [NBP2-41677] - HeLa cells (15 ug) were analyzed by WB blot using the H3 Pan antibody.")
| Immunogen | KLH-conjugated synthetic peptide of Histone H3 containing an unmodified sequence from the central part and from the C-terminus of histone H3. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | H3C1 |
| Purity | Whole Antiserum |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.05% Sodium Azide |
| Purity | Whole Antiserum |
Secondary Antibodies |
Isotype Controls |
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![Chromatin Immunoprecipitation (ChIP): HIST1H3A Antibody [NBP2-41677] - ChIP assays were performed using human osteosarcoma (U2OS) cells and the antibody and optimized PCR primer sets for qPCR. A titration of the antibody consisting of 2, 5, 10 and 15 uL per ChIP experiment was analyzed. IgG (5 ug/IP) was used as negative control.](http://images.novusbio.com/fullsize/HIST1H3A-C-Term-Antibody-Chromatin-Immunoprecipitation-ChIP-NBP2-41677-img0004.jpg "Chromatin Immunoprecipitation (ChIP): HIST1H3A Antibody [NBP2-41677] - ChIP assays were performed using human osteosarcoma (U2OS) cells and the antibody and optimized PCR primer sets for qPCR. A titration of the antibody consisting of 2, 5, 10 and 15 uL per ChIP experiment was analyzed. IgG (5 ug/IP) was used as negative control.")
![ELISA: HIST1H3A (C-Term) Antibody [NBP2-41677] - By plotting the absorbance against the antibody dilution the titer of the antibody was estimated to be 1:90,000.](http://images.novusbio.com/fullsize/HIST1H3A-C-Term-Antibody-ELISA-NBP2-41677-img0002.jpg "ELISA: HIST1H3A (C-Term) Antibody [NBP2-41677] - By plotting the absorbance against the antibody dilution the titer of the antibody was estimated to be 1:90,000.")
![Dot Blot: HIST1H3A (C-Term) Antibody [NBP2-41677] - Analysis was performed to test the cross reactivity of the antibody with peptides containing other modifications of histone H3 100 to 0.2 pmol of the peptide containing the respective histone modification were spotted on a membrane.](http://images.novusbio.com/fullsize/HIST1H3A-C-Term-Antibody-Dot-Blot-NBP2-41677-img0005.jpg "Dot Blot: HIST1H3A (C-Term) Antibody [NBP2-41677] - Analysis was performed to test the cross reactivity of the antibody with peptides containing other modifications of histone H3 100 to 0.2 pmol of the peptide containing the respective histone modification were spotted on a membrane.")
