![Western Blot: HIST1H3A [Monomethyl Lys4] Antibody [NBP2-41657] - Analysis of whole cell (25 ug, lane 1) and histone extracts (15 ug, lane 2) from HeLa cells, and on 1 ug of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively).](http://images.novusbio.com/fullsize/HIST1H3A-[Monomethyl-Lys4]-Antibody-Western-Blot-NBP2-41657-img0005.jpg "Western Blot: HIST1H3A [Monomethyl Lys4] Antibody [NBP2-41657] - Analysis of whole cell (25 ug, lane 1) and histone extracts (15 ug, lane 2) from HeLa cells, and on 1 ug of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively).")
| Immunogen | Polyclonal antibody raised in rabbit against histone H3 containing the monomethylated lysine 4 (H3K4me1), using a KLH-conjugated synthetic peptide. |
| Modification | Monomethyl Lys4 |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | H3C1 |
| Purity | Affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | PBS |
| Preservative | 0.05% Proclin 300 |
| Purity | Affinity purified |
![HIST1H3A [Monomethyl Lys4] Antibody](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "HIST1H3A [Monomethyl Lys4] Antibody")
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![Chromatin Immunoprecipitation: HIST1H3A [Monomethyl Lys4] Antibody [NBP2-41657] - ChIP assays were performed using 1 million HeLaS3 cells and the antibody and optimized PCR primer sets for qPCR. A titration of the antibody consisting of 1, 2, 5, and 10 ul per ChIP experiment was analysed. IgG (2 ug/IP) was used as negative control. The Fig shows the recovery, expressed as a % of input (the relative amount of IP DNA compared to input DNA after qPCR analysis).](http://images.novusbio.com/fullsize/HIST1H3A-Monomethyl-Lys4-Antibody-Chromatin-Immunoprecipitation-ChIP-NBP2-41657-img0009.jpg "Chromatin Immunoprecipitation: HIST1H3A [Monomethyl Lys4] Antibody [NBP2-41657] - ChIP assays were performed using 1 million HeLaS3 cells and the antibody and optimized PCR primer sets for qPCR. A titration of the antibody consisting of 1, 2, 5, and 10 ul per ChIP experiment was analysed. IgG (2 ug/IP) was used as negative control. The Fig shows the recovery, expressed as a % of input (the relative amount of IP DNA compared to input DNA after qPCR analysis).")
![ELISA: HIST1H3A [Monomethyl Lys4] Antibody [NBP2-41657] - The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution the titer of the antibody was estimated to be 1:20,000.](http://images.novusbio.com/fullsize/HIST1H3A-[Monomethyl-Lys4]-Antibody-ELISA-NBP2-41657-img0007.jpg "ELISA: HIST1H3A [Monomethyl Lys4] Antibody [NBP2-41657] - The antigen used was a peptide containing the histone modification of interest. By plotting the absorbance against the antibody dilution the titer of the antibody was estimated to be 1:20,000.")
![Dot Blot: HIST1H3A [Monomethyl Lys4] Antibody [NBP2-41657] - To test the cross reactivity of the antibody with peptides containing other modifications of histone H4 and H3. 100 to 0.2 pmol of the peptide containing the respective histone modification were spotted on a membrane. The Fig shows a high specificity of the antibody for the modification of interest.](http://images.novusbio.com/fullsize/HIST1H3A-[Monomethyl-Lys4]-Antibody-Dot-Blot-NBP2-41657-img0010.jpg "Dot Blot: HIST1H3A [Monomethyl Lys4] Antibody [NBP2-41657] - To test the cross reactivity of the antibody with peptides containing other modifications of histone H4 and H3. 100 to 0.2 pmol of the peptide containing the respective histone modification were spotted on a membrane. The Fig shows a high specificity of the antibody for the modification of interest.")
