HIST1H2BK [Monomethyl Arg79] Antibody (ST0430)

Immunocytochemistry/Immunofluorescence: HIST1H2BK [Monomethyl Arg79] Antibody (ST0430) [NBP2-67550] - Staining Histone H2B(mono methyl R79) in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: HIST1H2BK [Monomethyl Arg79] Antibody (ST0430) [NBP2-67550] - Analysis of paraffin-embedded mouse testis tissue using anti-Histone H2B(mono methyl R79) antibody. Counter stained with hematoxylin.

Immunocytochemistry/Immunofluorescence: HIST1H2BK [Monomethyl Arg79] Antibody (ST0430) [NBP2-67550] - Staining Histone H2B(mono methyl R79) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: HIST1H2BK [Monomethyl Arg79] Antibody (ST0430) [NBP2-67550] - Staining Histone H2B(mono methyl R79) in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: HIST1H2BK [Monomethyl Arg79] Antibody (ST0430) [NBP2-67550] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-Histone H2B(mono methyl R79) antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: HIST1H2BK [Monomethyl Arg79] Antibody (ST0430) [NBP2-67550] - Analysis of paraffin-embedded human tonsil tissue using anti-Histone H2B(mono methyl R79) antibody. Counter stained with hematoxylin.

Product Discontinued

HIST1H2BK [Monomethyl Arg79] Antibody (ST0430) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human HIST1H2BK aa 51-100 / 126. (SwissProt: P06899 Human; SwissProt: O60814 Human; SwissProt: P33778 Human; SwissProt: P23527 Human; SwissProt: Q64524 Mouse)
• Modification: Monomethyl Arg79
• Localization: Nucleus, Chromosome.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: HIST1H2BK
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:1000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for HIST1H2BK [Monomethyl Arg79] Antibody (ST0430)

• H2B histone family, member T
• H2B K
• H2B/S
• H2BFAiii
• H2BFT
• H2bk
• HIRA-interacting protein 1
• HIRIP1
• histone cluster 1, H2bk
• histone family member
• histone H2B type 1-K
• MGC131989

Background

HIST1H2BK is 126 amino acids long, weighs approximately 14 kDa, and the gene codes for a part of a group of basic nuclear proteins called histones, which are responsible for nucleosome structure of the chromosomal fiber in eukaryotes. Current studies are being done on several diseases and disorders including systemic lupus erythematosus, lupus erythematosus, and immunodeficiency. HIST1H2BK has also been shown to have interactions with HIST1H4A, HIST1H4B, HIST1H4C, HIST1H4D, and HIST1H4E in pathways such as the telomere maintenance, nucleosome assembly, cell cycle, and systemic lupus erythematosus pathways.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HIST1H2BK