HIST1H2BK [ac Lys20] Antibody (SN72-09)

Immunocytochemistry/Immunofluorescence: HIST1H2BK [ac Lys20] Antibody (SN72-09) [NBP2-67682] - Staining Histone H2B(acetyl K20) in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: HIST1H2BK [ac Lys20] Antibody (SN72-09) [NBP2-67682] - Analysis of paraffin-embedded mouse kidney tissue using anti-Histone H2B(acetyl K20) antibody. Counter stained with hematoxylin.

Flow Cytometry: HIST1H2BK [ac Lys20] Antibody (SN72-09) [NBP2-67682] - Analysis of Hela cells with Histone H2B(acetyl K20) antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was use

Immunocytochemistry/Immunofluorescence: HIST1H2BK [ac Lys20] Antibody (SN72-09) [NBP2-67682] - Staining Histone H2B(acetyl K20) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: HIST1H2BK [ac Lys20] Antibody (SN72-09) [NBP2-67682] - Staining Histone H2B(acetyl K20) in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: HIST1H2BK [ac Lys20] Antibody (SN72-09) [NBP2-67682] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-Histone H2B(acetyl K20) antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: HIST1H2BK [ac Lys20] Antibody (SN72-09) [NBP2-67682] - Analysis of paraffin-embedded human colon cancer tissue using anti-Histone H2B(acetyl K20) antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: HIST1H2BK [ac Lys20] Antibody (SN72-09) [NBP2-67682] - Analysis of paraffin-embedded human colon tissue using anti-Histone H2B(acetyl K20) antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: HIST1H2BK [ac Lys20] Antibody (SN72-09) [NBP2-67682] - Analysis of paraffin-embedded human kidney tissue using anti-Histone H2B(acetyl K20) antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: HIST1H2BK [ac Lys20] Antibody (SN72-09) [NBP2-67682] - Analysis of paraffin-embedded human tonsil tissue using anti-Histone H2B(acetyl K20) antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: HIST1H2BK [ac Lys20] Antibody (SN72-09) [NBP2-67682] - Analysis of paraffin-embedded mouse colon tissue using anti-Histone H2B(acetyl K20) antibody. Counter stained with hematoxylin.

Product Discontinued

HIST1H2BK [ac Lys20] Antibody (SN72-09) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within human HIST1H2BK aa 1-49/126. (SwissProt: O60814 Human; SwissProt: P06899 Human; SwissProt: P23527 Human; SwissProt: P33778 Human; SwissProt: P57053 Human; SwissProt: P58876 Human; SwissProt: P62807 Human; SwissProt: Q16778 Human; SwissProt: Q5QNW6 Human; SwissProt: Q8N257 Human; SwissProt: Q93079 Human; SwissProt: Q99877 Human; SwissProt: Q99879 Human; SwissProt: Q99880 Human; Entrez Gene: 64647 Mouse; Entrez Gene: Q00729 Rat)
• Modification: ac Lys20
• Localization: Chromosome, Nucleosome core, Nucleus.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: HIST1H2BK
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:100-1:500
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:100-1:500
  • Immunoprecipitation
  • Western Blot 1:1000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for HIST1H2BK [ac Lys20] Antibody (SN72-09)

• H2B histone family, member T
• H2B K
• H2B/S
• H2BFAiii
• H2BFT
• H2bk
• HIRA-interacting protein 1
• HIRIP1
• histone cluster 1, H2bk
• histone family member
• histone H2B type 1-K
• MGC131989

Background

HIST1H2BK is 126 amino acids long, weighs approximately 14 kDa, and the gene codes for a part of a group of basic nuclear proteins called histones, which are responsible for nucleosome structure of the chromosomal fiber in eukaryotes. Current studies are being done on several diseases and disorders including systemic lupus erythematosus, lupus erythematosus, and immunodeficiency. HIST1H2BK has also been shown to have interactions with HIST1H4A, HIST1H4B, HIST1H4C, HIST1H4D, and HIST1H4E in pathways such as the telomere maintenance, nucleosome assembly, cell cycle, and systemic lupus erythematosus pathways.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HIST1H2BK