HIST1H2BC Antibody (CL5101) - BSA Free

Western Blot: HIST1H2BC Antibody (CL5101) [NBP2-61154] - Analysis of purified human recombinant histone H2B, H2A, H3 and H4.

Immunohistochemistry: HIST1H2BC Antibody (CL5101) [NBP2-61154] - Staining of human striated muscle shows strong nuclear positivity in striated muscle fibers.

Western Blot: HIST1H2BC Antibody (CL5101) [NBP2-61154] - Western blot analysis in human cell line HEL.

Western Blot: HIST1H2BC Antibody (CL5101) [NBP2-61154] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.

Immunohistochemistry: HIST1H2BC Antibody (CL5101) [NBP2-61154] - Staining of human fallopian tube shows strong nuclear immunoreactivity in glandular and stromal cells.

Immunohistochemistry: HIST1H2BC Antibody (CL5101) [NBP2-61154] - Staining of human duodenum shows strong nuclear immunoreactivity in glandular cells and in lamina propria cells.

Immunohistochemistry: HIST1H2BC Antibody (CL5101) [NBP2-61154] - Staining of human prostate shows moderate to strong nuclear positivity in glandular and smooth muscle cells.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clone: CL5101
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

HIST1H2BC Antibody (CL5101) - BSA Free Summary

• Description: Novus Biologicals Mouse HIST1H2BC Antibody (CL5101) - BSA Free (NBP2-61154) is a monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed using a synthetic peptide derived from P62807, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: HIST1H2BC
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:1000 - 1:2500
  • Immunohistochemistry-Paraffin 1:1000 - 1:2500
  • Western Blot 1 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Protein A purified

Alternate Names for HIST1H2BC Antibody (CL5101) - BSA Free

• H2B histone family, member K
• H2B/a
• H2B/g
• H2B/kH2B/h
• H2BFA
• H2BFG
• H2BFH
• H2BFKH2B/l
• H2BFL
• HIST1H2BC
• HIST1H2BE
• HIST1H2BF
• HIST1H2BG
• histone 1, H2bi
• histone cluster 1, H2bi
• histone H2B type 1-C/E/F/G/I
• Histone H2B.1 A
• Histone H2B.a
• Histone H2B.g
• Histone H2B.h
• Histone H2B.k
• Histone H2B.l

Background

HIST1H2BC is 126 amino acids long, weighs approximately 14 kDa, and the gene codes for a part of a group of basic nuclear proteins called histones, which are responsible for nucleosome structure of the chromosomal fiber in eukaryotes. Current studies are being done on several diseases and disorders including systemic lupus erythematosus, lupus erythematosus, hemochromatosis, and immunodeficiency. HIST1H2BC has also been shown to have interactions with HIST1H4A, HIST1H4B, HIST1H4C, HIST1H4D, and HIST1H4E in pathways such as the histone modification, signal transduction of Activin A, telomere maintenance, nucleosome assembly, cell cycle, and systemic lupus erythematosus pathways.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: HIST1H2BC