Heparan Sulfate 6-O-Sulfotransferase 3/HS6ST3 Antibody [Alexa Fluor® 350]

• Reactivity: Mu
• Applications: IHC
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 350

Heparan Sulfate 6-O-Sulfotransferase 3/HS6ST3 Antibody [Alexa Fluor® 350] Summary

• Immunogen: Chinese hamster ovary cell line CHO-derived recombinant mouse Heparan Sulfate 6-O-Sulfotransferase/HS6ST3
  Pro28-Trp470
  Accession # NP_056635
• Specificity: Detects mouse Heparan Sulfate 6-O-Sulfotransferase/HS6ST3 in direct ELISAs.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Heparan Sulfate 6-O-Sulfotransferase 3/HS6ST3 Antibody [Alexa Fluor® 350]

• DKFZp761K2315
• heparan sulfate 6-O-sulfotransferase 3
• HS6ST3

Background

HS6ST3 (Heparin sulfate 6-O-sulfotransferase 3) is a 60-62 kDa member of the sulfotransferase 6 family of enzymes. It is one of three 6-O sulfotransferase isoforms, all of which essentially perform the same function, but all of which are the products of distinct genes. HS6ST3 is known to sulfate the #6 position of glucosamine sulfate that is linked to a 2-O-sulfated iduronic acid in heparin sulfate. The molecule shows a temporal expression pattern, being found in postnatal cerebellar Purkinje cells and cerebral cortical pyramidal cells in layers II/III and V. Mouse HS6ST3 is a 470 amino acid (aa) type II transmembrane glycoprotein. It contains a short four aa cytoplasmic N-terminus coupled to an extended 443 aa lumenal domain (aa 28-470). The lumenal domain contains a stem region (aa 28-83) that mediates oligomerization and Golgi localization, plus a sulfotransferase enzyme domain (aa 142-409). Cleavage within the stem region by beta -secretase generates a soluble form of HS6ST3. Over aa 28-470, mouse HS6ST3 shares 99% and 95% aa identity with rat and human HS6ST3, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.