Recombinant Human Heparan Sulfate 3-O-Sulfotransferase 1/HS3ST1 His Protein

SDS-Page: Recombinant Human Heparan Sulfate 3-O-Sulfotransferase 1/HS3ST1 Protein [NBP2-23116]

• Reactivity: Hu
• Applications: PAGE
• Concentration: 0.25 mg/ml

Recombinant Human Heparan Sulfate 3-O-Sulfotransferase 1/HS3ST1 His Protein Summary

• Description: A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 21-307 of Human Heparan Sulfate 3-O-Sulfotransferase 1/HS3ST1
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSRPAELGQ QELLRKAGTL QDDVRDGVAP NGSAQQLPQT IIIGVRKGGT RALLEMLSLH PDVAAAENEV HFFDWEEHYS HGLGWYLSQM PFSWPHQLTV EKTPAYFTSP KVPERVYSMN PSIRLLLILR DPSERVLSDY TQVFYNHMQK HKPYPSIEEF LVRDGRLNVD YKALNRSLYH VHMQNWLRFF PLRHIHIVDG DRLIRDPFPE IQKVERFLKL SPQINASNFY FNKTKGFYCL RDSGRDRCLH ESKGRAHPQV DPKLLNKLHE YFHEPNKKFF ELVGRTFDWH

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: HS3ST1
• Purity: >90%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 36.2 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH 8.0), 0.2 M NaCl, 40% glycerol, 2 mM DTT
• Preservative: No Preservative
• Concentration: 0.25 mg/ml
• Purity: >90%, by SDS-PAGE

Alternate Names for Recombinant Human Heparan Sulfate 3-O-Sulfotransferase 1/HS3ST1 His Protein

• 3OST
• 3OST1
• 3-OST-1
• 3OST1Heparan sulfate 3-O-sulfotransferase 1
• EC 2.8.2
• EC 2.8.2.23
• h3-OST-1
• heparan sulfate (glucosamine) 3-O-sulfotransferase 1
• Heparan sulfate D-glucosaminyl 3-O-sulfotransferase 1
• heparan sulfate glucosamine 3-O-sulfotransferase 1
• heparin-glucosamine 3-O-sulfotransferase
• HS3ST1

Background

Heparan sulfate glucosamine 3-O-sulfotransferase 1, also known as HS3ST1, is sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) to catalyze the transfer of a sulfo group to position 3 of glucosamine residues in heparan. This protein catalyzes the rate limiting step in the biosynthesis of heparan sulfate (HSact). This modification is a crucial step in the biosynthesis of anticoagulant heparan sulfate as it completes the structure of the antithrombin pentasaccharide binding site. Recombinant human HS3ST1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: HS3ST1