GRK2 Antibody (5D5)

Immunocytochemistry/Immunofluorescence: GRK2 Antibody (5D5) [NB300-156] - NB 300-156 (green) versus neurofilament NF-M (red, NB 300-134), in rat cortex, showing mostly large pyramidal neurons. Photo courtesy of Dr. Gerry Shaw, University of Florida.

Product Discontinued

GRK2 Antibody (5D5) Summary

• Immunogen: Purified recombinant human GRK2. [UniProt# P25098]
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: GRK2
• Purity: Tissue culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry 1:100
  • Western Blot 1:100
• Application Notes: This GRK2 antibody is useful for Immunohistochemistry and Western Blot. This antibody specifically recognizes purified recombinant GRK2 on immunoblots. It is particularly effective for the examination of the Immunocytochemical analysis of the expression of GRK2 in cells and tissue.
  
  The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Theoretical MW: 79.6 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Tissue culture supernatant
• Preservative: No Preservative
• Purity: Tissue culture supernatant

Alternate Names for GRK2 Antibody (5D5)

• ADRBK1
• adrenergic, beta, receptor kinase 1
• BARK
• BARK1
• BARK1beta-ARK-1
• Beta-ARK1
• Beta-ARK-1
• EC 2.7.11
• EC 2.7.11.15
• FLJ16718
• G-protein coupled receptor kinase 2
• GRK2
• GRK2beta-adrenergic receptor kinase 1

Background

GPCR kinases (GRKs) promote GPCR desensitization through receptor phosphorylation and subsequent beta-arrestin mediated receptor uncoupling from heterotrimeric G-protein. GRK2 is a widely expressed GRK that regulates phosphorylation/subsequent desensitization of multiple GPCRs, a critical regulatory process which is essential to signaling attenuation and receptors internalization mediated prevention of cellular over-stimulation. GRK2 also regulates signal transduction through direct interaction with p38 MAPK, MEK1/2, PI3K and the cAMP sensor Epac and its substrates also include certain cytoskeletal proteins including tubulin, radixin and ezrin. Among all GRK family members, GRK2 is unique in regulating growth and development, and the individuals without GRK2 are not viable. In vivo growth promoting activity of GRK2 has been ascribed to kinase-dependent/independent effects of GRK2 involving Smoothened (SMO) and Patched homolog 1. Besides its physiological role in growth and development, extravagant activity of GRK2 is a significant pathophysiological hallmark of cardiovascular problems.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: GRK2
• Entrez:
  • 156(Human)
  • 25238(Rat)
• Uniprot:
  • P25098()