GPR88 Antibody (1039830) [Unconjugated]

GPR88 was detected in immersion fixed paraffin-embedded sections of human brain (caudate nucleus) using Mouse Anti-Human GPR88 Monoclonal Antibody (Catalog # MAB10828) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies and processes. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

• Reactivity: Hu
• Applications: IHC
• Clone: 1039830
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

GPR88 Antibody (1039830) [Unconjugated] Summary

• Immunogen: Human GPR88 synthetic peptide
• Specificity: Detects human GPR88 in direct ELISAs.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GPR88 Antibody (1039830) [Unconjugated]

• G protein coupled receptor 88
• G protein-coupled receptor 88
• GPR88
• G-protein coupled receptor 88
• probable G-protein coupled receptor 88
• STRG
• Striatum-specific G-protein coupled receptor

Background

GPR88 is an orphan receptor that probably belongs to the G-protein coupled receptor family.  Ligands for this receptor still largely remain unknown but agonists have been discovered.  It is a brain-specific receptor with almost exclusive expression in the striatum, being preferentially localized in medium spiny neurons.  Because of its expression, GPR88 is emerging as a drug target for CNS disorders such as schizophrenia, Parkinson's disease, anxiety, and addiction. Gpr88 KO mice data suggests that striatal GPR88 receptors influence activity in a range of procedures integrated by prefrontal, orbitofrontal and anterior cingulate cortico-striatal-thalamic loops leading to altered cognitive, motivational and reward evaluation processes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.