GPR85 Antibody (1042202) [Unconjugated]

GPR85 was detected in immersion fixed U‑87 MG human glioblastoma/astrocytoma cell line (positive staining) and A549 human lung carcinoma cell line (negative staining) using Mouse Anti-Human GPR85 Monoclonal Antibody (Catalog # MAB10761) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

• Reactivity: Hu
• Applications: ICC/IF
• Clone: 1042202
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

GPR85 Antibody (1042202) [Unconjugated] Summary

• Immunogen: Human GPR85 extracellular domain synthetic peptide
• Specificity: Detects human GPR85 in cell based ELISAs.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 8-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GPR85 Antibody (1042202) [Unconjugated]

• FLJ50993
• FLJ51159
• FLJ99433
• G protein-coupled receptor 85
• GPR85
• probable G-protein coupled receptor 85
• seven transmembrane helix receptor
• SREB
• SREB2
• SREB2Super conserved receptor expressed in brain 2

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.