GNA14 Antibody (2H8)

Western Blot: GNA14 Antibody (2H8) [H00009630-M06] - Analysis of GNA14 expression in transfected 293T cell line by GNA14 monoclonal antibody (M06), clone 2H8. Lane 1: GNA14 transfected lysatE (41.6 KDa). Lane 2: Non-transfected lysate.

Immunohistochemistry-Paraffin: GNA14 Antibody (2H8) [H00009630-M06] - Analysis of monoclonal antibody to GNA14 on formalin-fixed paraffin-embedded human breast. Antibody concentration 3 ug/ml

ELISA: GNA14 Antibody (2H8) [H00009630-M06] - Detection limit for recombinant GST tagged GNA14 is 0.3 ng/ml as a capture antibody.

Product Discontinued

GNA14 Antibody (2H8) Summary

• Immunogen: GNA14 (AAH27886.1, 150 a.a. ~ 250 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. SDSAKYYLTDIDRIATPSFVPTQQDVLRVRVPTTGIIEYPFDLENIIFRMVDVGGQRSERRKWIHCFESVTSIIFLVALSEYDQVLAECDNENRMEESKAL
• Specificity: This antibody cross-reacts with human GNA11.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: GNA14
• Purity: Ascites

Applications/Dilutions

• Dilutions:
  • ELISA 1:100 - 1:2000
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:10 - 1:500
  • Western Blot 1:100 - 1:2000

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: Ascites

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for GNA14 Antibody (2H8)

• G alpha-14
• G-protein subunit alpha-14
• guanine nucleotide binding protein (G protein), alpha 14
• guanine nucleotide-binding protein 14
• guanine nucleotide-binding protein subunit alpha-14

Background

GNA14, also known as Guanine nucleotide-binding protein subunit alpha-14, is a 355 amino acid that is 42 kDa, engaged as modulator or transducer in various transmembrane signaling systems. Disease research is currently being studied with relation to GNA14 and acanthocytosis fainting, chorea, pertussis, chagas disease, trypanosomiasis, thrombosis, hypertension, hypertension, susceptibility to neuronitis, neuroblastoma, and pancreatitis. This protein has been linked to many pathways such as development activation of ERK by Alpha-1 adrenergic receptors, molecular mechanisms of cancer, intracellular calcium signaling, visual cycle in retinal rods, CDK5 pathway, ERK5 signaling, GPCR downstream signaling, hemostasis, gastrin-CREB signalling pathway via PKC and MAPK, signaling by GPCR, signal amplification, Chagas disease (American trypanosomiasis), and amoebiasis pathways where it interacts with approx. 700 proteins including OPRL1, CHRM2, CCR1, CXCR1, and CXCR2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for GNA14 Antibody (H00009630-M06)(1)

Publication using H00009630-M06

• Zou QY, Zhao YJ, Zhou C et al. G Protein ? Subunit 14 Mediates Fibroblast Growth Factor 2-Induced Cellular Responses in Human Endothelial Cells. J Cell Physiol 2018-11-01 [PMID: 30387149]

Bioinformatics

• Gene Symbol: GNA14
• Uniprot:
  • AAH27886.1(Human)